BMRB Entry 25047

Title:
RRM-Peptide and RES Complex
Deposition date:
2014-06-25
Original release date:
2014-08-26
Authors:
Tripsianes, Konstantinos; Friberg, A.; Barrandon, C.; Seraphin, B.; Sattler, M.
Citation:

Citation: Tripsianes, Konstantinos; Friberg, A.; Barrandon, C.; Seraphin, B.; Sattler, M.. "A Novel RRM-Peptide Interaction in the RES Complex"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
RRM, polymer, 93 residues, 10207.3111 Da.
RES, polymer, 39 residues, 4209.7028 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Data sets:
Data typeCount
1H chemical shifts861
13C chemical shifts427
15N chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM1
2RES2

Entities:

Entity 1, RRM 93 residues - 10207.3111 Da.

1   GLYALAMETGLYASNGLUTYRLYSASPASN
2   ALATYRILETYRILEGLYASNLEUASNARG
3   GLULEUTHRGLUGLYASPILELEUTHRVAL
4   PHESERGLUTYRGLYVALPROVALASPVAL
5   ILELEUSERARGASPGLUASNTHRGLYGLU
6   SERGLNGLYPHEALATYRLEULYSTYRGLU
7   ASPGLNARGSERTHRILELEUALAVALASP
8   ASNLEUASNGLYPHELYSILEGLYGLYARG
9   ALALEULYSILEASPHISTHRPHETYRARG
10   PROLYSARG

Entity 2, RES 39 residues - 4209.7028 Da.

1   GLYALAMETGLYASNARGPHEALAILEMET
2   PROGLYSERARGTRPASPGLYVALHISARG
3   SERASNGLYPHEGLUGLULYSTRPPHEALA
4   LYSGLNASNGLUILEASNGLULYSLYS

Samples:

sample_new_1: RRM, [U-13C; U-15N], 0.5 mM; RES, [U-13C; U-15N], 0.5 mM; H2O 93%; D2O, [U-100% 2H], 7%

sample_conditions_new_1: ionic strength: 25.000 mM; pH: 6.300; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
15N-EDITED 3D NOESYsample_new_1solutionsample_conditions_new_1
13C- EDITED 3D NOESY (ALIPHATICS)sample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (AROMATICS)sample_new_1solutionsample_conditions_new_1
13C- EDITED 3D NOESY (ALIPHATICS) F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1

Software:

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

CcpNmr_Analysis v2.1, CCPN - chemical shift assignment

TALOS vany, Cornilescu, Delaglio and Bax - chemical shift assignment

NMR spectrometers:

  • BRUKER AVANCE 800 MHz

Related Database Links:

UNP IST3_YEAST CWC26_YEAST
BMRB 19766 25442 25443
PDB
DBJ GAA23224
EMBL CAA59179 CAA96886 CAY79593
GB AAS56830 AHY79214 EDN61950 EDV10384 EDZ72293
REF NP_011341
SP P46947
TPG DAA07939
AlphaFold D6VVT5 D6VTX8 P46947

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks