BMRB Entry 20109

Title:
CHARACTERIZATION OF THE LIGAND-FREE CONSTITUTIVE ACTIVITY OF THE THROMBOXANE A2 RECEPTOR BY THE INTEGRATED APPROACHES OF NMR SPECTROSCOPY AND A RECOMBINANT RECEPTOR
Deposition date:
2009-12-09
Original release date:
2011-02-14
Authors:
Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He
Citation:

Citation: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He. "Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity"  Biochemistry 49, 6365-6374 (2010).
PubMed: 20590159

Assembly members:

Assembly members:
Gaq-Ct peptide, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gaq-Ct peptide: KDTILQLNLKEYNLV

Data sets:
Data typeCount
1H chemical shifts104
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Gaq-Ct peptide1

Entities:

Entity 1, Gaq-Ct peptide 15 residues - Formula weight is not available

1   LYSASPTHRILELEUGLNLEUASNLEULYS
2   GLUTYRASNLEUVAL

Samples:

sample_1: Gaq-Ct peptide 1.1 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

FELIX v2000, Accelrys Software Inc. - chemical shift assignment, chemical shift calculation, data analysis, peak picking, processing, refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 20112 20118
EMBL CAG08422
GB ACF47673 ACF47675 EGW10015 EGW13773 EHB02791
REF XP_004048208 XP_004059801 XP_005431300 XP_005642455 XP_008508489