BMRB Entry 20012

Title:
Tertiary structure and lipid interactions of short recombinant antimicrobial peptide
Deposition date:
2008-03-21
Original release date:
2009-04-23
Authors:
Japelj, Bostjan
Citation:

Citation: Zorko, Mateja; Japelj, Bostjan; Hafner-Bratkovica, Iva; Jerala, Roman. "Expression, purification and structural studies of a short antimicrobial peptide"  Biochim. Biophys. Acta 1788, 314-323 (2009).
PubMed: 19026609

Assembly members:

Assembly members:
PFR_peptide, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PFR_peptide: PFWRIRIRRX

Data sets:
Data typeCount
1H chemical shifts84
conformer coordinate sets1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PFR peptide1

Entities:

Entity 1, PFR peptide 10 residues - Formula weight is not available

1   PROPHETRPARGILEARGILEARGARGNH2

Samples:

sample_1: PFR peptide 1.8 mM; SDS mM

sample_conditions_1: pH: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

DYANA, Guntert, Braun and Wuthrich - geometry optimization

NMR spectrometers:

  • Varian INOVA 600 MHz