BMRB Entry 19677

Title:
Solution structure of the CLAVATA-like encoded peptide of Meloidogyne hapla - MhCLE4
Deposition date:
2013-12-13
Original release date:
2014-12-22
Authors:
Bobay, Benjamin; DiGennaro, Peter; Bird, David
Citation:

Citation: DiGennaro, Peter; Bobay, Benjamin; Bird, David. "Inferring function of CLE peptides from high resolution tertiary structures"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
entity, polymer, 12 residues, 1222.254 Da.

Natural source:

Natural source:   Common Name: nematode   Taxonomy ID: 6305   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meloidogyne hapla

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: HEVPSGPNPSSN

Data sets:
Data typeCount
13C chemical shifts23
15N chemical shifts11
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CLAVATA-like encoded peptide of Meloidogyne hapla - MhCLE41

Entities:

Entity 1, CLAVATA-like encoded peptide of Meloidogyne hapla - MhCLE4 12 residues - 1222.254 Da.

1   HISGLUVALPROSERGLYPROASNPROSER
2   SERASN

Samples:

sample_1: entity 4 mg/mL; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 140 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks