BMRB Entry 19438

Title:
tgam
Deposition date:
2013-08-19
Original release date:
2014-09-02
Authors:
Yang, Yinshan; Montet de Guillen, Karine; Roumestand, Christian
Citation:

Citation: Yang, Yinshan; Montet de Guillen, Karine; Roumestand, Christian. "NMR structure of Tgam"  .

Assembly members:

Assembly members:
Tgam, polymer, 148 residues, 16758.439 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Ecoli

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts136
1H chemical shifts295

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tgam1

Entities:

Entity 1, Tgam 148 residues - 16758.439 Da.

1   METLYSTYRASPVALVALILEILEPROGLU
2   SERPHEHISARGPHEASPLYSHISASNMET
3   GLUHISILECYSPROPROMETVALILEGLY
4   ASPARGSERTYRASPILEALAMETGLUILE
5   VALASNGLYVALASPARGVALILELYSALA
6   SERPHEASNALASERVALGLUGLULEUGLU
7   GLYGLUASPCYSASPVALLEUTYRARGLYS
8   TYRTHRLEUGLULYSGLUGLYLYSLYSGLY
9   ILEVALHISVALLYSLEUARGLYSILETHR
10   GLUASNCYSPROPROVALASPGLYASNARG
11   CYSSERVALLEUGLUPHEGLUARGASPILE
12   GLUCYSILEVALLYSALAILEGLUGLUCYS
13   LEUALALYSGLYGLULEUASNSERLYSLEU
14   GLUGLYLYSPROILEPROASNPROLEULEU
15   GLYLEUASPSERTHRARGTHRGLY

Samples:

sample_1: H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.60; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB ACS34436
REF WP_015859542

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks