BMRB Entry 19252

Title:
RRM domain of HUMAN RBM7
Deposition date:
2013-05-21
Original release date:
2015-01-26
Authors:
Sikorsky, Tomas; Hrossova, Dominika; Vanacova, Stepanka; Stefl, Richard
Citation:

Citation: Sikorsky, Tomas; Stefl, Richard; Hrossova, Dominika; Vanacova, Stepanka. "RNA binding properties of the human NEXT complex"  .

Assembly members:

Assembly members:
RBM7, polymer, 101 residues, 11473.301 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-SMT3

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts80
1H chemical shifts602

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM domain of HUMAN RBM71

Entities:

Entity 1, RRM domain of HUMAN RBM7 101 residues - 11473.301 Da.

1   GLYSERGLUPHEGLULEUARGARGGLNGLN
2   ALALEUALAGLUALAASPARGTHRLEUPHE
3   VALGLYASNLEUGLUTHRLYSVALTHRGLU
4   GLULEULEUPHEGLULEUPHEHISGLNALA
5   GLYPROVALILELYSVALLYSILEPROLYS
6   ASPLYSASPGLYLYSPROLYSGLNPHEALA
7   PHEVALASNPHELYSHISGLUVALSERVAL
8   PROTYRALAMETASNLEULEUASNGLYILE
9   LYSLEUTYRGLYARGPROILELYSILEGLN
10   PHEARGSERGLYSERSERHISALAPROGLN
11   ASP

Samples:

sample_1: RBM7, [U-99% 13C; U-99% 15N], 0.3 mM; BISTRIS 20 mM; beta-mercaptoethanol 4 mM; sodium chloride 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB
DBJ BAA92036 BAD92990 BAG35505 BAG73694
EMBL CAH93091
GB AAD39257 AAH34381 AAH70262 ADQ31580 AIC50533
REF NP_001248000 NP_001272974 NP_057174 XP_002754472 XP_002822554
SP Q9Y580
AlphaFold Q9Y580

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks