BMRB Entry 19216

Title:
Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2
Deposition date:
2013-05-02
Original release date:
2013-06-04
Authors:
Ullah, Asad; Kemp, Grant; Lee, Brian; Alves, Claudia; Young, Howard; Sykes, Brian; Fliegel, Larry
Citation:

Citation: Ullah, Asad; Kemp, Grant; Lee, Brian; Alves, Claudia; Young, Howard; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2."  J. Biol. Chem. 288, 24609-24624 (2013).
PubMed: 23836910

Assembly members:

Assembly members:
sod2_TM_IV, polymer, 38 residues, 3073.673 Da.

Natural source:

Natural source:   Common Name: Fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMal-c2x

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sod2_TM_IV: GSKKKLFPQINFLGSLLIAG CITSTDPVLSALIVGKKK

Data sets:
Data typeCount
15N chemical shifts35
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sod2_TM_IV1

Entities:

Entity 1, sod2_TM_IV 38 residues - 3073.673 Da.

1   GLYSERLYSLYSLYSLEUPHEPROGLNILE
2   ASNPHELEUGLYSERLEULEUILEALAGLY
3   CYSILETHRSERTHRASPPROVALLEUSER
4   ALALEUILEVALGLYLYSLYSLYS

Samples:

sample_1: sod2_TM_IV, [U-99% 15N], 0.2 – 0.7 mM; CDCl3 50 v/v; 2-propanol 50 v/v

sample_conditions_1: pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESY-HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSY-HSQCsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ, Johnson, One Moon Scientific - data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks