BMRB Entry 19187

Title:
1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Methanothermobacter thermautotrophicus
Deposition date:
2013-04-24
Original release date:
2013-09-30
Authors:
Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias
Citation:

Citation: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias. "(1)H, (15)N and (13)C chemical shift assignments for the winged helix domains of two archeal MCM C-termini."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23934138

Assembly members:

Assembly members:
C-terminus_MCM, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanothermobacter thermautotrophicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX HTa

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts83
1H chemical shifts645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Methanothermobacter thermautotrophicus MCM C-terminus1

Entities:

Entity 1, Methanothermobacter thermautotrophicus MCM C-terminus 88 residues - Formula weight is not available

1   GLYALAMETGLYGLUTHRGLYLYSILEASP
2   ILEASPLYSVALGLUGLYARGTHRPROLYS
3   SERGLUARGASPLYSPHEARGLEULEULEU
4   GLULEUILELYSGLUTYRGLUASPASPTYR
5   GLYGLYARGALAPROTHRASNILELEUILE
6   THRGLUMETMETASPARGTYRASNVALSER
7   GLUGLULYSVALGLUGLULEUILEARGILE
8   LEULYSASPLYSGLYALAILEPHEGLUPRO
9   ALAARGGLYTYRLEULYSILEVAL

Samples:

sample_1: C-terminus of the minichromosome maintenance protein MCM of Methanothermobacter thermautotrophicus, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; H20 90%; D2O 10%

sample_2: C-terminus of the minichromosome maintenance protein MCM of Methanothermobacter thermautotrophicus, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; D2O 100%

sample_conditions_1: ionic strength: 160 mM; pH: 6.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CcpNMR, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AvanceIII 750 MHz

Related Database Links:

UniProtKB O27798
PDB
GB AAB86236
REF WP_048061327

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks