BMRB Entry 18949

Title:
The H/D-exchange Kinetics of the Escherichia coli Co-chaperonin GroES studied by 2D-NMR and DMSO-Quenched Exchange Methods
Deposition date:
2013-01-14
Original release date:
2013-06-04
Authors:
Chandak, Mahesh; Nakamura, Takashi; Makabe, Koki; Takenaka, Toshio; Mukaiyama, Atsushi; Chaudhuri, Tapan; Chen, Jin; Kato, Koichi; Kuwajima, Kunihiro
Citation:

Citation: Chandak, Mahesh; Nakamura, Takashi; Makabe, Koki; Takenaka, Toshio; Mukaiyama, Atsushi; Chaudhuri, Tapan; Kato, Koichi; Kuwajima, Kunihiro. "The H/D-Exchange Kinetics of the Escherichia coli Co-Chaperonin GroES Studied by 2D NMR and DMSO-Quenched Exchange Methods."  J. Mol. Biol. 425, 2541-2560 (2013).
PubMed: 23583779

Assembly members:

Assembly members:
GroES, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. Coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PETESWILD

Data sets:
Data typeCount
15N chemical shifts43
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GroES_11
2GroES_21
3GroES_31

Entities:

Entity 1, GroES_1 97 residues - Formula weight is not available

1   METASNILEARGPROLEUHISASPARGVAL
2   ILEVALLEUARGLEUGLUVALGLUTHRLYS
3   SERALAGLYGLYILEVALLEUTHRGLYSER
4   ALAALAALALYSSERTHRARGGLYGLUVAL
5   LEUALAVALGLYASNGLYARGILELEUGLU
6   ASNGLYGLUVALLEUPROLEUASPVALLEU
7   VALGLYASPILEVALILEPHEASNASPGLY
8   TYRGLYVALLEUSERGLULEUILEASPASN
9   GLUGLUVALASPILEMETSERGLUSERASP
10   ILELEUALAILEVALGLUALA

Samples:

sample_1: DMSO, [U-100% 15N], 300 uM; D2O 95%; H2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availableisotropicsample_conditions_1

Software:

NMRView, One Moon Scientific,INC (NIH) - chemical shift assignment

NMR spectrometers:

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks