BMRB Entry 18768

Title:
Structural study of NS2(2-32) GBVB protein
Deposition date:
2012-10-08
Original release date:
2014-04-07
Authors:
Montserret, Roland; Penin, Francois; Martin, Annette
Citation:

Citation: Boukadida, Celia; Marnata, Caroline; Montserret, Roland; Cohen, Lisette; Blumen, Brigitte; Gouttenoire, Jerome; Moradpour, Darius; Penin, Francois; Martin, Annette. "NS2 proteins of GB virus B and hepatitis C virus share common protease activities and membrane topologies."  J. Virol. 88, 7426-7444 (2014).
PubMed: 24741107

Assembly members:

Assembly members:
NS2_2-32_GBVB, polymer, 31 residues, 3370.069 Da.

Natural source:

Natural source:   Common Name: Hepatitis GB virus B   Taxonomy ID: 39113   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: none   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NS2_2-32_GBVB: DTEIIGGLTIPPVVALVVMS RFGFFAHLLPR

Data sets:
Data typeCount
13C chemical shifts102
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS2_2-32_GBVB1

Entities:

Entity 1, NS2_2-32_GBVB 31 residues - 3370.069 Da.

1   ASPTHRGLUILEILEGLYGLYLEUTHRILE
2   PROPROVALVALALALEUVALVALMETSER
3   ARGPHEGLYPHEPHEALAHISLEULEUPRO
4   ARG

Samples:

sample_1: NS2_2-32_GBVB, [U-99% 2H], 2 ± 0.1 mM; TFE 50 % v/v; H2O 50 % v/v

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis, structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz