BMRB Entry 18766

Title:
Spatial stucture of PI-AnmTX Ugr 9a-1
Deposition date:
2012-10-08
Original release date:
2013-07-01
Authors:
Mineev, Konstantin; Arseniev, Alexander
Citation:

Citation: Osmakov, Dmitry; Kozlov, Sergey; Andreev, Yaroslav; Koshelev, Sergey; Sanamyan, Nadezhda; Sanamyan, Karen; Dyachenko, Igor; Bondarenko, Dmitry; Murashev, Arkadii; Mineev, Konstantin; Arseniev, Alexander; Grishin, Eugene. "Sea anemone peptide with uncommon -hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity."  J. Biol. Chem. 288, 23116-23127 (2013).
PubMed: 23801332

Assembly members:

Assembly members:
UGTX, polymer, 29 residues, 3142.515 Da.

Natural source:

Natural source:   Common Name: sea anemones   Taxonomy ID: 45620   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Urticina grebelnyi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UGTX: ISIDPPCRFCYHRDGSGNCV YDAYGCGAV

Data sets:
Data typeCount
13C chemical shifts77
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PI-AnmTX Ugr 9a-11

Entities:

Entity 1, PI-AnmTX Ugr 9a-1 29 residues - 3142.515 Da.

1   ILESERILEASPPROPROCYSARGPHECYS
2   TYRHISARGASPGLYSERGLYASNCYSVAL
3   TYRASPALATYRGLYCYSGLYALAVAL

Samples:

sample_1: UGTX 2 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0.3, Cornilescu, Delaglio and Bax - collection, processing

CARA v1.9.4, Keller and Wuthrich - chemical shift assignment, data analysis

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - data analysis, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB