BMRB Entry 18287

Title:
Backbone and sidechain 1H chemical shifts for PAP248-286 (SEVI) in solution at pH 6
Deposition date:
2012-02-21
Original release date:
2012-03-22
Authors:
Popovych, Nataliya; Brender, Jeffrey; Soong, Ronald; Vivekanandan, Subramanian; Hartman, Kevin; Basrur, Venkatesha; MacDonald, Peter; Ramamoorthy, Ayyalusamy
Citation:

Citation: Popovych, Nataliya; Brender, Jeffrey; Soong, Ronald; Vivekanandan, Subramanian; Hartman, Kevin; Basrur, Venkatesha; Macdonald, Peter; Ramamoorthy, Ayyalusamy. "Site specific interaction of the polyphenol EGCG with the SEVI amyloid precursor peptide PAP(248-286)."  J. Phys. Chem. B 116, 3650-3658 (2012).
PubMed: 22360607

Assembly members:

Assembly members:
PAP248-286, polymer, 39 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PAP248-286: GIHKQKEKSRLQGGVLVNEI LNHMKRATQIPSYKKLIMY

Data sets:
Data typeCount
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAP248-2861

Entities:

Entity 1, PAP248-286 39 residues - Formula weight is not available

1   GLYILEHISLYSGLNLYSGLULYSSERARG
2   LEUGLNGLYGLYVALLEUVALASNGLUILE
3   LEUASNHISMETLYSARGALATHRGLNILE
4   PROSERTYRLYSLYSLEUILEMETTYR

Samples:

sample_1: PAP248-286 0.3 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 17193 17346 17347 17924 17925
PDB
DBJ BAD89417 BAG62248
EMBL CAA36422 CAA37673
GB AAA60021 AAA60022 AAA69694 AAB60640 AAH07460
REF NP_001090 NP_001127666 NP_001278966 XP_001115549 XP_001148736
SP P15309
AlphaFold P15309