BMRB Entry 18162

Title:
Neurotensin 40 structures in water pH 5.5 298 K. NMR data & structures
Deposition date:
2011-12-27
Original release date:
2012-08-16
Authors:
Mukhopadhyay, Chaitali; Khatun, Ummul Liha
Citation:

Citation: Khatun, Ummul; Goswami, Sudipto; Mukhopadhyay, Chaitali. "Modulation of the neurotensin solution structure in the presence of ganglioside GM1 bicelle"  Biophys. Chem. 168, 48-59 (2012).
PubMed: 22824320

Assembly members:

Assembly members:
entity, polymer, 26 residues, 3369.949 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Plasmid

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: ELYENKPRRPYILELYENKP RRPYIL

Data sets:
Data typeCount
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neurotensin1

Entities:

Entity 1, Neurotensin 26 residues - 3369.949 Da.

1   GLULEUTYRGLUASNLYSPROARGARGPRO
2   TYRILELEUGLULEUTYRGLUASNLYSPRO
3   ARGARGPROTYRILELEU

Samples:

sample_1: Neurotensin 4.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 4.5 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Charles Schwieters, T.D. Goddard, D.G. Kneller - chemical shift assignment, processing, refinement

SPARKY, Charles Schwieters, T.D. Goddard, D.G. Kneller - chemical shift assignment, processing, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 18163 18164