BMRB Entry 17948

Title:
NMR structure of Hsp12 in the presence of DPC
Deposition date:
2011-09-20
Original release date:
2011-10-28
Authors:
Singarapu, Kiran; Markley, John
Citation:

Citation: Singarapu, Kiran; Tonelli, Marco; Chow, Darius; Frederick, Ronnie; Westler, William; Markley, John. "Structural characterization of Hsp12, the heat shock protein from Saccharomyces cerevisiae, in aqueous solution where it is intrinsically disordered and in detergent micelles where it is locally -helical."  J. Biol. Chem. 286, 43447-43453 (2011).
PubMed: 21998307

Assembly members:

Assembly members:
Hsp12, polymer, 109 residues, 11712.830 Da.

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pvp68k

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts102
1H chemical shifts537

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsp121

Entities:

Entity 1, Hsp12 109 residues - 11712.830 Da.

1   METSERASPALAGLYARGLYSGLYPHEGLY
2   GLULYSALASERGLUALALEULYSPROASP
3   SERGLNLYSSERTYRALAGLUGLNGLYLYS
4   GLUTYRILETHRASPLYSALAASPLYSVAL
5   ALAGLYLYSVALGLNPROGLUASPASNLYS
6   GLYVALPHEGLNGLYVALHISASPSERALA
7   GLULYSGLYLYSASPASNALAGLUGLYGLN
8   GLYGLUSERLEUALAASPGLNALAARGASP
9   TYRMETGLYALAALALYSSERLYSLEUASN
10   ASPALAVALGLUTYRVALSERGLYARGVAL
11   HISGLYGLUGLUASPPROTHRLYSLYS

Samples:

sample_1: Hsp12, [U-13C; U-15N], 0.7 mM; DPC 200 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

VNMR, Varian - collection

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17482 17483 18523
PDB
DBJ BAA09224 BAA14033 GAA23069
EMBL CAA39306 CAA86349 CAY79436
GB AAA34647 AAL06077 AAS56790 AHY75775 AJP38487
REF NP_116640
SP P22943
TPG DAA12425
AlphaFold P22943

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks