BMRB Entry 17936

Title:
N/A
Deposition date:
2011-09-13
Original release date:
2012-01-09
Authors:
Barraud, Pierre; Allain, Frederic
Citation:

Citation: Barraud, Pierre; Heale, Bret; Allain, Mary. "Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA."  Biochimie 94, 1499-1509 (2012).
PubMed: 22210494

Assembly members:

Assembly members:
dADAR-dsRBD1, polymer, 93 residues, 10262.124 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts263
15N chemical shifts75
1H chemical shifts559

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dADAR-dsRBD11

Entities:

Entity 1, dADAR-dsRBD1 93 residues - 10262.124 Da.

1   SERASPPROLYSLYSLYSMETCYSLYSGLU
2   ARGILEPROGLNPROLYSASNTHRVALALA
3   METLEUASNGLULEUARGHISGLYLEUILE
4   TYRLYSLEUGLUSERGLNTHRGLYPROVAL
5   HISALAPROLEUPHETHRILESERVALGLU
6   VALASPGLYGLNLYSTYRLEUGLYGLNGLY
7   ARGSERLYSLYSVALALAARGILEGLUALA
8   ALAALATHRALALEUARGSERPHEILEGLN
9   PHELYSASPGLYALAVALLEUSERPROLEU
10   LYSPROALA

Samples:

sample_15N: dADAR-dsRBD1, [U-15N], 0.8 – 1.2 mM; H2O 90%; D2O 10%

sample_13C15N: dADAR-dsRBD1, [U-13C; U-15N], 0.8 – 1.2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_15Nisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_13C15Nisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_13C15Nisotropicsample_conditions_1
3D HNCAsample_13C15Nisotropicsample_conditions_1
3D HNCACBsample_13C15Nisotropicsample_conditions_1
3D CBCA(CO)NHsample_13C15Nisotropicsample_conditions_1
3D HCCH-TOCSYsample_13C15Nisotropicsample_conditions_1
3D H(CCO)NHsample_13C15Nisotropicsample_conditions_1
3D 1H-15N NOESYsample_15Nisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_13C15Nisotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_13C15Nisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v2.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
EMBL CAA22774
GB AAF45665 AAF63702 AAF63703 AAK26850 AAN09057
REF NP_001033821 NP_001245469 NP_001245470 NP_001245471 NP_001245472
SP Q9NII1
AlphaFold Q9NII1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks