BMRB Entry 17825

Title:
Solution Structure of the J Domain of HSJ1a
Deposition date:
2011-08-02
Original release date:
2012-01-09
Authors:
Zhou, Chenjie; Gao, Xuechao; Cao, Chunyang; Hu, Hongyu
Citation:

Citation: Gao, Xue-Chao; Zhou, Chen-Jie; Zhou, Zi-Ren; Wu, Meng; Cao, Chun-Yang; Hu, Hong-Yu. "The C-terminal helices of heat shock protein 70 are essential for J-domain binding and ATPase activation."  J. Biol. Chem. 287, 6044-6052 (2012).
PubMed: 22219199

Assembly members:

Assembly members:
entity, polymer, 99 residues, 8556.679 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts185
15N chemical shifts66
1H chemical shifts367

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HSJ1a1

Entities:

Entity 1, HSJ1a 99 residues - 8556.679 Da.

1   METALASERTYRTYRGLUILELEUASPVAL
2   PROARGSERALASERALAASPASPILELYS
3   LYSALATYRARGARGLYSALALEUGLNTRP
4   HISPROASPLYSASNPROASPASNLYSGLU
5   PHEALAGLULYSLYSPHELYSGLUVALALA
6   GLUALATYRGLUVALLEUSERASPLYSHIS
7   LYSARGGLUILETYRASPARGTYRGLYARG
8   GLUPROLEUTHRGLYTHRGLYTHRGLYPRO
9   SERARGALAGLUALAGLYSERGLYGLYPRO
10   GLYLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: HSJ1a, [U-100% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_2: HSJ1a, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - peak picking

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAE87866 BAE88406 BAF82315 BAF85450 BAG35597
EMBL CAA44968 CAA44969
GB AAA09034 AAA09035 AAH11609 AAH47056 AAP35751
REF NP_001034639 NP_001162567 NP_001253510 NP_001267342 NP_001271020
SP P25686
AlphaFold P25686

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks