BMRB Entry 17813

Title:
NMR structure of the UHRF1 PHD domain
Deposition date:
2011-07-28
Original release date:
2011-09-01
Authors:
Wang, Chengkun
Citation:

Citation: Wang, Chengkun; Shen, Jie; Yang, Zhongzheng; Chen, Ping; Zhao, Bin; Hu, Wei; Lan, Wenxian; Tong, Xiaotian; Wu, Houming; Li, Guohong; Cao, Chunyang. "Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger."  Cell Res. 21, 1379-1382 (2011).
PubMed: 21808299

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, 7849.913 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts64
1H chemical shifts376

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UHRF1 PHD domain1
2ZINC ION_12
3ZINC ION_22
4ZINC ION_32

Entities:

Entity 1, UHRF1 PHD domain 69 residues - 7849.913 Da.

1   SERGLYPROSERCYSLYSHISCYSLYSASP
2   ASPVALASNARGLEUCYSARGVALCYSALA
3   CYSHISLEUCYSGLYGLYARGGLNASPPRO
4   ASPLYSGLNLEUMETCYSASPGLUCYSASP
5   METALAPHEHISILETYRCYSLEUASPPRO
6   PROLEUSERSERVALPROSERGLUASPGLU
7   TRPTYRCYSPROGLUCYSARGASNASP

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 1.5 mM; sodium chloride 137 mM; potassium phosphate 10 mM; DTT 3 mM; potassium chloride 2.7 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17808 17812
PDB
DBJ BAF36719 BAF36720 BAF82078 BAG37156
GB AAF28469 AAI13876 AAK55744 AAV40831 ABQ59043
REF NP_001041666 NP_001276979 NP_001276980 NP_001276981 NP_037414
SP Q96T88
AlphaFold Q96T88

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks