BMRB Entry 17772

Title:
Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)
Deposition date:
2011-07-08
Original release date:
2011-12-06
Authors:
Kappo, M.; Ab, E.; Atkinson, R.; Faro, A.; Muleya, V.; Mulaudzi, T.; Poole, J.; Mckenzie, J.; PUGH, DAVID
Citation:

Citation: Kappo, Mautin; Ab, Eiso; Hassem, Faqeer; Atkinson, R. Andrew; Faro, Andrew; Muleya, Victor; Mulaudzi, Takalani; Poole, John; McKenzie, Jean; Chibi, Moredreck; Moolman-Smook, Joanna; Rees, D. Jasper G; Pugh, David. "Solution Structure of RING Finger-like Domain of Retinoblastoma-binding Protein-6 (RBBP6) Suggests It Functions as a U-box."  J. Biol. Chem. 287, 7146-7158 (2012).
PubMed: 22130672

Assembly members:

Assembly members:
E3_UBIQUITIN-PROTEIN_LIGASE_RBBP6, polymer, 92 residues, 10214.4517 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts96
1H chemical shifts631

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E3 UBIQUITIN-PROTEIN LIGASE RBBP6 11
2E3 UBIQUITIN-PROTEIN LIGASE RBBP6 21
3Zinc ion, 12
4Zinc ion, 22

Entities:

Entity 1, E3 UBIQUITIN-PROTEIN LIGASE RBBP6 1 92 residues - 10214.4517 Da.

1   GLYPROLEUGLYSERGLUASPASPPROILE
2   PROASPGLULEULEUCYSLEUILECYSLYS
3   ASPILEMETTHRASPALAVALVALILEPRO
4   CYSCYSGLYASNSERTYRCYSASPGLUCYS
5   ILEARGTHRALALEULEUGLUSERASPGLU
6   HISTHRCYSPROTHRCYSHISGLNASNASP
7   VALSERPROASPALALEUILEALAASNLYS
8   PHELEUARGGLNALAVALASNASNPHELYS
9   ASNGLUTHRGLYTYRTHRLYSARGLEUARG
10   LYSGLN

Entity 2, Zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: E3 UBIQUITIN-PROTEIN LIGASE RBBP6, [U-13C; U-15N], 0.7 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQC-NOESY 13C-HSQC-NOESYsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS v2.1, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMRView vany, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UNP RBBP6_HUMAN
BMRB 11159 17466
PDB
DBJ BAC77636 BAC77637 BAG64162
EMBL CAA59445
GB AAB49620 AAC72432 AAH63524 AAI72357 AAL05625
REF NP_001291483 NP_008841 NP_035377 NP_061173 XP_001076339
SP P97868 Q7Z6E9
TPG DAA15466
AlphaFold Q9NPX4 Q7Z6E9 P97868

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks