BMRB Entry 17752

Name: SOLUTION STRUCTURE OF THE HUMAN PROLACTIN RECEPTOR ECD DOMAIN D2
Published: 2012-02-13

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PDB ID: 2lfg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17752
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE HUMAN PROLACTIN RECEPTOR ECD DOMAIN D2

Deposition date:

2011-06-30

Original release date:

2012-02-13

Authors:

Dagil, Robert; Knudsen, Maiken; Kragelund, Birthe; O'Shea, Charlotte

Citation:

Citation: Dagil, Robert; Knudsen, Maiken; Olsen, Johan; O'Shea, Charlotte; Franzmann, Magnus; Goffin, Vincent; Teilum, Kaare; Breinholt, Jens; Kragelund, Birthe. "The WSXWS Motif in Cytokine Receptors Is a Molecular Switch Involved in Receptor Activation: Insight from Structures of the Prolactin Receptor" Structure 20, 270-282 (2012).

Assembly members:

Assembly members:
hPRLR-D2, polymer, 113 residues, 13328.4 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hPRLR-D2: MYIVQPDPPLELAVEVKQPE DRKPYLWIKWSPPTLIDLKT GWFTLLYEIRLKPEKAAEWE IHFAGQQTEFKILSLHPGQK YLVQVRCKPDHGYWSAWSPA TFIQIPSDFTMND

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	549
15N chemical shifts	112
1H chemical shifts	809

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hPRLR-D2	1

Entities:

Entity 1, hPRLR-D2 113 residues - 13328.4 Da.

1

MET

TYR

ILE

VAL

GLN

PRO

ASP

PRO

LEU

2

GLU

LEU

ALA

VAL

GLU

VAL

LYS

GLN

PRO

GLU

3

ASP

ARG

LYS

PRO

TYR

LEU

TRP

ILE

LYS

TRP

4

SER

PRO

THR

LEU

ILE

ASP

LEU

LYS

THR

5

GLY

TRP

PHE

THR

LEU

TYR

GLU

ILE

ARG

6

LEU

LYS

PRO

GLU

LYS

ALA

GLU

TRP

GLU

7

ILE

HIS

PHE

ALA

GLY

GLN

THR

GLU

PHE

8

LYS

ILE

LEU

SER

LEU

HIS

PRO

GLY

GLN

LYS

9

TYR

LEU

VAL

GLN

VAL

ARG

CYS

LYS

PRO

ASP

10

HIS

GLY

TYR

TRP

SER

ALA

TRP

SER

PRO

ALA

11

THR

PHE

ILE

GLN

ILE

PRO

SER

ASP

PHE

THR

12

MET

ASN

ASP

Samples:

sample_1: hPRLR-D2, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 10 mM; TCEP 10 mM; DSS 1 mM; sodium azide 0.02%; D2O 10%; H2O 90%

sample_2: hPRLR-D2, [U-100% 13C(1)], 0.5 mM; sodium phosphate 10 mM; TCEP 10 mM; sodium azide 0.02%; DSS 1 mM; D2O 100%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CYANA v2.2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

X-PLOR NIH v2.25, Schwieters, Kuszewski, Tjandra and Clore - structure solution

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN v2.1.5, CCPN - peak picking

ARIA v2.3.2, Linge, O'Donoghue and Nilges - Water refinement

NMR spectrometers:

Varian INOVA 800 MHz
Bruker Avance 800 MHz

PDB	1BP3 2LFG 3D48 3MZG 3N06 3N0P 3NCB 3NCC 3NCE 3NCF 4I18
DBJ	BAG36079 BAG73583
EMBL	CAH91100 CAL38328 CAL38451
GB	AAA60174 AAB34470 AAD32032 AAD49855 AAH59392
REF	NP_000940 NP_001153263 NP_001191243 NP_001191244 NP_001191245
SP	P16471
AlphaFold	P16471