BMRB Entry 17334

Name: EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1
Published: 2012-06-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17334

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1

Deposition date:

2010-12-02

Original release date:

2012-06-06

Authors:

Smallridge, Rachel; Whiteman, Pat; Doering, Klaus; Handford, Penny; Downing, A.

Citation:

Citation: Smallridge, Rachel; Whiteman, Pat; Doering, Klaus; Handford, Penny; Downing, A.. "EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1" J. Mol. Biol. 286, 661-668 (1999).
PubMed: 10024441

Assembly members:

Assembly members:
EGF, polymer, 86 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EGF: TDIDECRISPDLCGRGQCVN TPGDFECKCDEGYESGFMMM KNCMDIDECQRDPLLCRGGV CHNTEGSYRCECPPGHQLSP NISACI

Data sets:

binding_constants

Data type	Count
binding constants	2

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cbEGF12	1
2	cbEGF13	1
3	CA	2

Entities:

Entity 1, cbEGF12 86 residues - Formula weight is not available

1

THR

ASP

ILE

ASP

GLU

CYS

ARG

ILE

SER

PRO

2

ASP

LEU

CYS

GLY

ARG

GLY

GLN

CYS

VAL

ASN

3

THR

PRO

GLY

ASP

PHE

GLU

CYS

LYS

CYS

ASP

4

GLU

GLY

TYR

GLU

SER

GLY

PHE

MET

5

LYS

ASN

CYS

MET

ASP

ILE

ASP

GLU

CYS

GLN

6

ARG

ASP

PRO

LEU

CYS

ARG

GLY

VAL

7

CYS

HIS

ASN

THR

GLU

GLY

SER

TYR

ARG

CYS

8

GLU

CYS

PRO

GLY

HIS

GLN

LEU

SER

PRO

9

ASN

ILE

SER

ALA

CYS

ILE

Entity 2, CA - Ca - 40.078 Da.

1

CA

Samples:

sample_1: EGF 131 uM; sodium chloride 150 mM; Tris 4.55 mM; calcium chloide0 – 277.72 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 306 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	isotropic	sample_conditions_1

Software:

software -

NMR spectrometers:

GE Omega 600 MHz