BMRB Entry 17297

Title:
NMR Solution structure of GLP-2 in DHPC micelles
Deposition date:
2010-11-12
Original release date:
2011-03-30
Authors:
Venneti, Kalyana; Hewage, Chandralal
Citation:

Citation: Venneti, Kalyana; Hewage, Chandralal. "Conformational and molecular interaction studies of glucagon-like peptide-2 with its N-terminal extracellular receptor domain."  FEBS Lett. 585, 346-352 (2011).
PubMed: 21167157

Assembly members:

Assembly members:
GLP-2, polymer, 33 residues, 3769.167 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GLP-2: HADGSFSDEMNTILDNLAAR DFINWLIQTKITD

Data sets:
Data typeCount
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GLP-21

Entities:

Entity 1, GLP-2 33 residues - 3769.167 Da.

1   HISALAASPGLYSERPHESERASPGLUMET
2   ASNTHRILELEUASPASNLEUALAALAARG
3   ASPPHEILEASNTRPLEUILEGLNTHRLYS
4   ILETHRASP

Samples:

sample_1: DHPC 350 mM

sample_conditions_1: pH: 3.9; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 17296
PDB
GB EAX11358
REF XP_002749449 XP_002812569 XP_003266221 XP_003309324 XP_003820986