BMRB Entry 17291

Title:
A novel design concept: New Y-receptor agonists with increased membrane recruitment, Y2 affinity and selectivity
Deposition date:
2010-11-11
Original release date:
2014-03-04
Authors:
Pedersen, Soren; Jurt, Simon; Zerbe, Oliver; Jensen, Knud
Citation:

Citation: Pedersen, Soren; Bhatia, Vikrram; Jurt, Simon; Pedersen, Maria; Stamou, Dimitrios; Zerbe, Oliver; Holst, Brigitte; Vrang, Niels; Jensen, Knud. "Improving membrane binding as a design strategy for amphipathic peptide hormones: 2-helix variants of PYY3-36"  J. Pept. Sci. 18, 579-587 (2012).
PubMed: 22865741

Assembly members:

Assembly members:
entity_1, polymer, 41 residues, 5104.971 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: YLERELKKLERELKKLSPEE LNRYYASLRHYLNLVTRQRY

Data sets:
Data typeCount
1H chemical shifts323

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PYY1

Entities:

Entity 1, PYY 41 residues - 5104.971 Da.

1   TYRLEUGLUARGGLULEULYSLYSLEUGLU
2   ARGGLULEULYSLYSLEUSERPROGLUGLU
3   LEUASNARGTYRTYRALASERLEUARGHIS
4   TYRLEUASNLEUVALTHRARGGLNARGTYR

Samples:

sample_1: PYY 1 mM; DPC 300 mM; MES 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, P.GUNTERT ET AL. - structure solution

AMBER v6, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

XEASY v1.2, Bartels C, Xia TH, Billeter M, Guentert P, Wuethrich K - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB