BMRB Entry 17000

Name: Structure of E1-69 of Yeast V-ATPase
Published: 2011-03-24

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PDB ID: 2kz9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17000
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of E1-69 of Yeast V-ATPase

Deposition date:

2010-06-14

Original release date:

2011-03-24

Authors:

Sankaranarayanan, Rishikesan; Gruber, Gerhard

Citation:

Citation: Rishikesan, Sankaranarayanan; Thaker, Youg; Gruber, Gerhard. "NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase." J. Bioenerg. Biomembr. 43, 187-193 (2011).
PubMed: 21399923

Assembly members:

Assembly members:
entity, polymer, 69 residues, 7908.008 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MASAITALTPNQVNDELNKM QAFIRKEAEEKAKEIQLKAD QEYEIEKTNIVRNETNNIDG NFKSKLKKA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	202
15N chemical shifts	68
1H chemical shifts	427

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E1-69	1

Entities:

Entity 1, E1-69 69 residues - 7908.008 Da.

1

MET

ALA

SER

ALA

ILE

THR

ALA

LEU

THR

PRO

2

ASN

GLN

VAL

ASN

ASP

GLU

LEU

ASN

LYS

MET

3

GLN

ALA

PHE

ILE

ARG

LYS

GLU

ALA

GLU

4

LYS

ALA

LYS

GLU

ILE

GLN

LEU

LYS

ALA

ASP

5

GLN

GLU

TYR

GLU

ILE

GLU

LYS

THR

ASN

ILE

6

VAL

ARG

ASN

GLU

THR

ASN

ILE

ASP

GLY

7

ASN

PHE

LYS

SER

LYS

LEU

LYS

ALA

Samples:

sample_1: protein, [U-100% 13C; U-100% 15N], 2 mM; Na2PO4 25 mM; NaCl 200 mM; EDTA 5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 200 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, P.GUNTERT ET AL. - refinement

Molmol, Koradi, Billeter and Wuthrich - structure solution

SPARKY, Goddard - peak picking

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2KZ9 3J9T 3J9U 3J9V 4DL0 4EFA
DBJ	GAA26638
EMBL	CAA89978 CAA99654 CAY86613
GB	AAA35209 AHY77605 AJP41835 AJT71258 AJT71746
REF	NP_014977
SP	P22203
TPG	DAA11094
AlphaFold	P22203