BMRB Entry 16963

Title:
Structure of the scorpion toxin U1-Liotoxin-Lw1a
Deposition date:
2010-05-28
Original release date:
2012-03-29
Authors:
Smith, Jennifer; Hill, Justine; Alewood, Paul; King, Glenn
Citation:

Citation: Smith, Jennifer; Hill, Justine; Little, Michelle; Nicholson, Graham; King, Glenn; Alewood, Paul. "Unique scorpion toxin with a putative ancestral fold provides insight into evolution of the inhibitor cystine knot motif"  Proc. Natl. Acad. Sci. U. S. A. 108, 10478-10483 (2011).
PubMed: 21670253

Assembly members:

Assembly members:
U1-Liotoxin-Lw1a, polymer, 36 residues, 4174.9 Da.

Natural source:

Natural source:   Common Name: Australian rainforest scorpion   Taxonomy ID: 27399   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Liocheles waigiensis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U1-Liotoxin-Lw1a: DFPLSKEYESCVRPRKCKPP LKCNKAQICVDPNKGW

Data sets:
Data typeCount
1H chemical shifts253

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1U1-Liotoxin-Lw1a1

Entities:

Entity 1, U1-Liotoxin-Lw1a 36 residues - 4174.9 Da.

1   ASPPHEPROLEUSERLYSGLUTYRGLUSER
2   CYSVALARGPROARGLYSCYSLYSPROPRO
3   LEULYSCYSASNLYSALAGLNILECYSVAL
4   ASPPROASNLYSGLYTRP

Samples:

sample_1: LITX 0.3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 1 mM; pH: 3.1; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
SP P0DJ08
AlphaFold P0DJ08