BMRB Entry 16851

Title:
The structure of E-protein activation domain 1 bound to the KIX domain of CBP/p300 elucidates leukemia induction by E2A-PBX1
Deposition date:
2010-04-08
Original release date:
2012-08-02
Authors:
Denis, Christopher; Chitayat, Seth; Plevin, Michael; Liu, Shuang; Spencer, Holly; Ikura, Mitsuhiko; LeBrun, David; Smith, Steven
Citation:

Citation: Denis, Christopher; Langelaan, David; Kirlin, Alyssa; Chitayat, Seth; Munro, Kim; Spencer, Holly; LeBrun, David; Smith, Steven. "Functional redundancy between the transcriptional activation domains of E2A is mediated by binding to the KIX domain of CBP/p300."  Nucleic Acids Res. ., .-. (2014).
PubMed: 24682819

Assembly members:

Assembly members:
KIX, polymer, 87 residues, 10353.954 Da.
ETAD1, polymer, 19 residues, 2021.198 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21a(+)

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts112
1H chemical shifts656

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIX1
2ETAD12

Entities:

Entity 1, KIX 87 residues - 10353.954 Da.

1   GLYVALARGLYSGLYTRPHISGLUHISVAL
2   THRGLNASPLEUARGSERHISLEUVALHIS
3   LYSLEUVALGLNALAILEPHEPROTHRPRO
4   ASPPROALAALALEULYSASPARGARGMET
5   GLUASNLEUVALALATYRALALYSLYSVAL
6   GLUGLYASPMETTYRGLUSERALAASNSER
7   ARGASPGLUTYRTYRHISLEULEUALAGLU
8   LYSILETYRLYSILEGLNLYSGLULEUGLU
9   GLULYSARGARGSERARGLEU

Entity 2, ETAD1 19 residues - 2021.198 Da.

1   GLYSERGLYTHRASPLYSGLULEUSERASP
2   LEULEUASPPHESERALAMETPHESER

Samples:

sample_KIX_ETAD1_1: KIX 1.2 mM; ETAD1, [U-13C; U-15N], 0.6 mM; DSS 1 mM; MES 20 mM; beta-mercaptoethanol 1 mM

sample_KIX_ETAD1_2: KIX, [U-13C; U-15N], 0.6 mM; ETAD1 1.2 mM; DSS 1 mM; MES 20 mM; beta-mercaptoethanol 1 mM

condition_KIX_ETAD1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
2D 1H-13C HSQCsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D CBCA(CO)NHsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D C(CO)NHsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D HNCOsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D HNCACBsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D HCCH-TOCSYsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D H(CCO)NHsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D 1H-13C NOESYsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1
3D 1H-15N NOESYsample_KIX_ETAD1_1isotropiccondition_KIX_ETAD1

Software:

NMRPipe v2.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TOPSPIN, Bruker Biospin - collection

NMRView v5.2.2, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18314 18315 18694 18695 16467
PDB
DBJ BAE06125 BAG65526 BAI45616 BAC26095 BAC38116 BAE21912 BAE21989 BAE25324
GB AAB28651 AAC08447 AAC51331 AAC51770 AAH72594 AAA42115 AAA58632 AAA60311 AAB32662 AAB62389
PRF 1923401A 2208360B
REF NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371 NP_001071353 NP_001077431 NP_001077436 NP_001187118 NP_001187247
SP P45481 Q6JHU9 Q92793 P15884 P51514 Q60722 Q61286 Q99081
TPG DAA15549 DAA01129 DAA25320
EMBL CAA46052 CAA62868 CAD89914 CAF92364 CAG00972
AlphaFold P45481 Q6JHU9 Q92793 Q99081 Q61286 Q60722 P51514 P15884

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks