BMRB Entry 16769

Title:
Solution structure of the par toxin Fst in DPC micelles
Deposition date:
2010-03-08
Original release date:
2010-08-12
Authors:
Zangger, Klaus; Gobl, Christoph; Kosol, Simone; Rueckert, Hanna Marie
Citation:

Citation: Gobl, Christoph; Kosol, Simone; Stockner, Thomas; Ruckert, Hanna; Zangger, Klaus. "Solution structure and membrane binding of the toxin fst of the par addiction module."  Biochemistry 49, 6567-6575 (2010).
PubMed: 20677831

Assembly members:

Assembly members:
Fst, polymer, 33 residues, 3767.501 Da.

Natural source:

Natural source:   Common Name: E. faecalis   Taxonomy ID: 1351   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fst: MKDLMSLVIAPIFVGLVLEM ISRVLDEEDDSRK

Data sets:
Data typeCount
1H chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fst1

Entities:

Entity 1, Fst 33 residues - 3767.501 Da.

1   METLYSASPLEUMETSERLEUVALILEALA
2   PROILEPHEVALGLYLEUVALLEUGLUMET
3   ILESERARGVALLEUASPGLUGLUASPASP
4   SERARGLYS

Samples:

sample_1: Fst_toxin .5 mM; potassium phosphate 50 mM; DPC, [U-99% 2H], 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian Unity 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
GB AAF03910 EEI55805 EEN72987 EEU27112 EOE77546
REF WP_002387930 YP_009074128