BMRB Entry 16316

Title:
NMR structure of IntB phage-integrase-like protein fragment 90-199 from Erwinia carotova subsp. atroseptica: Northeast Structural Genomics Consortium target EwR217E
Deposition date:
2009-05-26
Original release date:
2012-08-03
Authors:
Cort, John; Ramelot, Theresa; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Nair, Rajesh; Rost, Burkhard; Swapna, G.V.T.; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Cort, John; Montelione, Gaetano; Kennedy, Michael. "NMR structure of IntB phage-integrase-like protein fragment 90-199 from Erwinia carotova subsp. atroseptica"  .

Assembly members:

Assembly members:
fragment 90-199, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacterium carotovorum   Taxonomy ID: 554   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Erwinia carotovora

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21-23C

Data sets:
Data typeCount
13C chemical shifts505
15N chemical shifts122
1H chemical shifts778

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fragment 90-1991

Entities:

Entity 1, fragment 90-199 118 residues - Formula weight is not available

1   LYSSERVALGLNGLULYSARGASNASNTHR
2   ARGALAPHELYSTHRVALALALYSSERTRP
3   PHEALATHRLYSTHRTHRTRPSERGLUASP
4   TYRGLNARGSERVALTRPTHRARGLEUGLU
5   THRTYRLEUPHEPROASPILEGLYASNLYS
6   ASPILEALAGLULEUASPTHRGLYASPLEU
7   LEUVALPROILELYSLYSILEGLULYSLEU
8   GLYTYRLEUGLUILEALAMETARGVALLYS
9   GLNTYRALATHRALAILEMETARGTYRALA
10   VALGLNGLNLYSMETILEARGPHEASNPRO
11   ALATYRASPLEUGLUGLYALAVALGLNLYS
12   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 1 mM; MES 20 mM; sodium chloride 200 mM; calcium chloride 5 mM; sodium azide 0.02 w/v; H2O 95%; D2O, [U-99% 2H], 5%

sample_2: entity, [5% biosynthetically directed 13C; U-99% 15N], 0.7 mM; MES 20 mM; sodium chloride 200 mM; calcium chloride 5 mM; sodium azide 0.02 w/v; H2O 95%; D2O, [U-99% 2H], 5%

sample_3: entity, [U-99% 13C; U-99% 15N], 0.7 mM; MES 20 mM; sodium chloride 200 mM; calcium chloride 5 mM; sodium azide 0.02 w/v; D2O, [U-99% 2H], 100%

sample_conditions_1: ionic strength: 215 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
4D 1H-13C-13C-1H HMQC-NOESY-HMQCsample_3isotropicsample_conditions_1

Software:

AutoStruct, Huang, Tejero, Powers and Montelione - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

SPARKY, Goddard - chemical shift assignment, data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks