BMRB Entry 15647

Title:
1H chemical shift and NMR structure of Herpes simplex virus type 1 (HSV-1)fusion peptide gH626-644
Deposition date:
2008-02-03
Original release date:
2008-08-22
Authors:
Raiola, Luca; Falanga, Annarita; Isernia, Carla; Fattorusso, Roberto; Galdiero, Stefania; Pedone, Carlo
Citation:

Citation: Galdiero, Stefania; Falanga, Annarita; Vitiello, Mariateresa; Raiola, Luca; Fattorusso, Roberto; Browne, Helena; Pedone, Carlo; Isernia, Carla; Galdiero, Massimiliano. "Analysis of a membrane interacting region of herpes simplex virus type 1 glycoprotein H"  J. Biol. Chem. 283, 29993-30009 (2008).
PubMed: 18678872

Assembly members:

Assembly members:
HSV-1_gH_protein,_gH626-644_fusion_peptide, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 10298   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simplexvirus Human herpesvirus 1

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HSV-1_gH_protein,_gH626-644_fusion_peptide: GLASTLTRWAHYNALIRAF

Data sets:
Data typeCount
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HSV-1 gH protein, gH626-644 fusion peptide1

Entities:

Entity 1, HSV-1 gH protein, gH626-644 fusion peptide 19 residues - Formula weight is not available

1   GLYLEUALASERTHRLEUTHRARGTRPALA
2   HISTYRASNALALEUILEARGALAPHE

Samples:

gH626-644: TFE, [U-99% 2H], 80 v/v; H2O 20 v/v; gH626-644 2 mM

gH626-644_conditions: pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYgH626-644isotropicgH626-644_conditions
2D 1H-1H NOESYgH626-644isotropicgH626-644_conditions
2D DQF-COSYgH626-644isotropicgH626-644_conditions

Software:

XEASY,CYANA, Bartels et al., Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 16699 18335
PDB
DBJ BAM73371
EMBL CAA27534 CAA32335
GB AAA45815 AAG17895 ABI63484 ACM62244 ADD59995
REF YP_009137096
SP P06477 P08356 Q9DHD5
AlphaFold P06477 Q9DHD5 P08356