BMRB Entry 15503

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for MxiM(28-142)
Deposition date:
2007-10-02
Original release date:
2008-10-31
Authors:
Okon, Mark; Lario, Paula; Creagh, Louise; Jung, Young; Maurelli, Anthony; Strynadka, Natalie; McIntosh, Lawrence
Citation:

Citation: Okon, Mark; Moraea, Trevor; Lario, Paula; Creagh, Louise; Haynes, Charles; Strynadka, Natalie; McIntosh, Lawrence. "Structural characterization of the type-III pilot-secretin complex from Shigella flexneri"  Structure 16, 1544-1554 (2008).
PubMed: 18940609

Assembly members:

Assembly members:
MxiM(28-142), polymer, 115 residues, 12650 Da.

Natural source:

Natural source:   Common Name: Shigella flexneri   Taxonomy ID: 623   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shigella flexneri

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts216
15N chemical shifts105
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MxiM(28-142)1

Entities:

Entity 1, MxiM(28-142) 115 residues - 12650 Da.

1   SERSERSERASNSERGLULYSGLUTRPHIS
2   ILEVALPROVALSERLYSASPTYRPHESER
3   ILEPROASNASPLEULEUTRPSERPHEASN
4   THRTHRASNLYSSERILEASNVALTYRSER
5   LYSCYSILESERGLYLYSALAVALTYRSER
6   PHEASNALAGLYLYSPHEMETGLYASNPHE
7   ASNVALLYSGLUVALASPGLYCYSPHEMET
8   ASPALAGLNLYSILEALAILEASPLYSLEU
9   PHESERMETLEULYSASPGLYVALVALLEU
10   LYSGLYASNLYSILEASNASPTHRILELEU
11   ILEGLULYSASPGLYGLUVALLYSLEULYS
12   LEUILEARGGLYILE

Samples:

sample_1: MxiM(28-142), [U-95% 13C; U-95% 15N], 0.3 -0.5 mM; HEPES 20 mM; dodecylmaltosid 0.5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView vnmrview5.0.4.linux, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 15504
PDB
DBJ BAA09152
EMBL CAC05818 CEP57531 CSE38496 CSE96731 CSF42817
GB AAA26534 AAK18462 AAL72325 AAP79006 AAZ91125
REF NP_085306 NP_858276 WP_001346200 WP_010921672 WP_039064510
SP P0A1X2 P0A1X3
AlphaFold P0A1X2 P0A1X3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks