BMRB Entry 15332

Title:
Solution sturcture of human MEKK3 PB1 domain cis isomer
Deposition date:
2007-06-27
Original release date:
2007-10-29
Authors:
Qi, Hu; Jiahai, Zhang; Jihui, Wu; Yunyu, Shi
Citation:

Citation: Qi, Hu; Weiqun, Shen; Hongda, Huang; Jiangxin, Liu; Jiahai, Zhang; Xiaojuan, Huang; Jihui, Wu; Yunyu, Shi. "Insight into the Binding Properties of MEKK3 PB1 to MEK5 PB1 from Its Solution Structure"  Biochemistry 46, 13478-13489 (2007).
PubMed: 17985933

Assembly members:

Assembly members:
MEKK3_PB1-cis, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts348
15N chemical shifts93
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MEKK3 PB1-cis1

Entities:

Entity 1, MEKK3 PB1-cis 94 residues - Formula weight is not available

1   METGLNSERASPVALARGILELYSPHEGLU
2   HISASNGLYGLUARGARGILEILEALAPHE
3   SERARGPROVALLYSTYRGLUASPVALGLU
4   HISLYSVALTHRTHRVALPHEGLYGLNPRO
5   LEUASPLEUHISTYRMETASNASNGLULEU
6   SERILELEULEULYSASNGLNASPASPLEU
7   ASPLYSALAILEASPILELEUASPARGSER
8   SERSERMETLYSSERLEUARGILELEULEU
9   LEUSERGLNASPARGASNLEUGLUHISHIS
10   HISHISHISHIS

Samples:

sample_1: MEKK3 PB1-cis, [U-100% 13C; U-100% 15N], 0.5 mM; phosphate buffer 50 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_2: MEKK3 PB1-cis, [U-100% 15N], 0.8 mM; phosphate buffer 50 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Koradi, Billeter and Wuthrich - data analysis, processing, structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

BMRB 15355
PDB
DBJ BAD90481 BAE23244 BAG37709 BAG73407
EMBL CAD38973 CAL37711
GB AAB03535 AAB41729 AAH08336 AAH23781 AAH90859
REF NP_001100528 NP_001244715 NP_002392 NP_036077 NP_976226
SP Q61084 Q99759
TPG DAA18328
AlphaFold Q61084 Q99759

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks