BMRB Entry 15295

Title:
Solution structure At3g28950.1 from Arabidopsis thaliana
Deposition date:
2007-06-07
Original release date:
2007-08-15
Authors:
Volkman, B.; de la Cruz, N.; Peterson, F.
Citation:

Citation: de la Cruz, N.; Peterson, F.; Volkman, B.. "Solution structure of At3g28950 from Arabidopsis thaliana"  Proteins 71, 546-551 (2008).
PubMed: 18214976

Assembly members:

Assembly members:
At3g28950.1, polymer, 165 residues, 18603.959 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pVP13

Data sets:
Data typeCount
13C chemical shifts612
15N chemical shifts149
1H chemical shifts929

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1At3g28950.11

Entities:

Entity 1, At3g28950.1 165 residues - 18603.959 Da.

1   SERTHRSERSERASPPROGLNSERHISASN
2   VALPHEVALTYRGLYSERILELEUGLUPRO
3   ALAVALALAALAVALILELEUASPARGTHR
4   ALAASPTHRVALPROALAVALLEUHISGLY
5   TYRHISARGTYRLYSLEULYSGLYLEUPRO
6   TYRPROCYSILEVALSERSERASPSERGLY
7   LYSVALASNGLYLYSVALILETHRGLYVAL
8   SERASPALAGLULEUASNASNPHEASPVAL
9   ILEGLUGLYASNASPTYRGLUARGVALTHR
10   VALGLUVALVALARGMETASPASNSERGLU
11   LYSVALLYSVALGLUTHRTYRVALTRPVAL
12   ASNLYSASPASPPROARGMETTYRGLYGLU
13   TRPASPPHEGLUGLUTRPARGVALVALHIS
14   ALAGLULYSPHEVALGLUTHRPHEARGLYS
15   METLEUGLUTRPASNLYSASNPROASNGLY
16   LYSSERMETGLUGLUALAVALGLYSERLEU
17   LEUSERSERGLYASP

Samples:

sample: At3g28950.1, [U-100% 13C; U-100% 15N], 0.75 mM; MOPS 10 mM; sodium chloride 100 mM; DTT 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

xwinnmr v3.5, Bruker - collection

NMRPipe v2004, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

SPSCAN v1.1.0, R.W. Glaser - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA95746 BAE99955
GB AAM64749 AAO42842 AEE77512
REF NP_189537

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks