BMRB Entry 11417

Title:
Solution structure of the J domain of DnaJ homolog subfamily B member 8
Deposition date:
2010-09-24
Original release date:
2010-10-08
Authors:
Ohnishi, S.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Ohnishi, S.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the J domain of DnaJ homolog subfamily B member 8"  .

Assembly members:

Assembly members:
DnaJ domain, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050613-27

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts87
1H chemical shifts554

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaJ domain1

Entities:

Entity 1, DnaJ domain 92 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETALAASN
2   TYRTYRGLUVALLEUGLYVALGLNALASER
3   ALASERPROGLUASPILELYSLYSALATYR
4   ARGLYSLEUALALEUARGTRPHISPROASP
5   LYSASNPROASPASNLYSGLUGLUALAGLU
6   LYSLYSPHELYSLEUVALSERGLUALATYR
7   GLUVALLEUSERASPSERLYSLYSARGSER
8   LEUTYRASPARGALAGLYCYSASPSERTRP
9   ARGALAGLYGLYGLYALASERGLYPROSER
10   SERGLY

Samples:

sample_1: DnaJ domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.965, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAE01188 BAG54269 BAG54285
GB AAH29521 AAH50288 ADQ32829 AIC53322 EAW79320
REF NP_001271507 NP_699161 XP_001097989 XP_005979495 XP_006713582
SP Q8NHS0
AlphaFold Q8NHS0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks