BMRB Entry 11366

Title:
Assigned chemical shifts of the human spliceosomal protein complex p14-SF3b155
Deposition date:
2010-09-08
Original release date:
2011-09-07
Authors:
Kuwasako, K.; Dohmae, N.; Inoue, M.; Shirouzu, M.; Guntert, P.; Seraphin, B.; Muto, Y.; Yokoyama, S.
Citation:

Citation: Kuwasako, K.; Dohmae, N.; Inoue, M.; Shirouzu, M.; Guntert, P.; Seraphin, B.; Muto, Y.; Yokoyama, S.. "NMR solution structure of the human spliceosomal protein complex p14-SF3b155"  .

Assembly members:

Assembly members:
spliceosomal protein SF3b155, residues in database 379-424, polymer, 47 residues, Formula weight is not available
spliceosomal protein p14, RNA recognition motif, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli   Vector: PGEX6P-1-SF3B155(379-424)-HIS6-P14(8-93)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
spliceosomal protein SF3b155, residues in database 379-424: GTPEQLQAWRWEREIDERNR PLSDEELDAMFPEGYKVLPP PAGYVPI
spliceosomal protein p14, RNA recognition motif: GRANIRLPPEVNRILYIRNL PYKITAEEMYDIFGKYGPIR QIRVGNTPETRGTAYVVYED IFDAKNACDHLSGFNVCNRY LVVLYYN

Data sets:
Data typeCount
13C chemical shifts516
15N chemical shifts112
1H chemical shifts804

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1spliceosomal protein SF3b155, residues in database 379-4241
2spliceosomal protein p14, RNA recognition motif2

Entities:

Entity 1, spliceosomal protein SF3b155, residues in database 379-424 47 residues - Formula weight is not available

1   GLYTHRPROGLUGLNLEUGLNALATRPARG
2   TRPGLUARGGLUILEASPGLUARGASNARG
3   PROLEUSERASPGLUGLULEUASPALAMET
4   PHEPROGLUGLYTYRLYSVALLEUPROPRO
5   PROALAGLYTYRVALPROILE

Entity 2, spliceosomal protein p14, RNA recognition motif 87 residues - Formula weight is not available

1   GLYARGALAASNILEARGLEUPROPROGLU
2   VALASNARGILELEUTYRILEARGASNLEU
3   PROTYRLYSILETHRALAGLUGLUMETTYR
4   ASPILEPHEGLYLYSTYRGLYPROILEARG
5   GLNILEARGVALGLYASNTHRPROGLUTHR
6   ARGGLYTHRALATYRVALVALTYRGLUASP
7   ILEPHEASPALALYSASNALACYSASPHIS
8   LEUSERGLYPHEASNVALCYSASNARGTYR
9   LEUVALVALLEUTYRTYRASN

Samples:

sample_1: spliceosomal protein SF3b155, residues in database 379-424 300 mM; spliceosomal protein p14, RNA recognition motif 300 mM; Sodium Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A - data analysis

Kujira v0.8995, Kobayashi, N - data analysis

CYANA v2.1, Guntert, P - refinement, structure solution

OPALP v1.4, Koradi, R. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 11367
PDB
DBJ BAB28369 BAB40140 BAE24537 BAE27592 BAB22052 BAB22936 BAE30577 BAG34849 BAI45933
EMBL CAA70201 CAG00132 CAJ82723 CDQ59950 CDQ82948
GB AAC97189 AAG01404 AAH03419 AAH15530 AAH28959 AAD34105 AAF29138 AAG09698 AAH15463 AAH19535
REF NP_001084150 NP_001179923 NP_001252892 NP_036565 NP_112456 NP_001002067 NP_001017249 NP_001039482 NP_001079940 NP_001153997
SP O57683 O75533 Q99NB9 P59708 Q9Y3B4
TPG DAA32571 DAA21891 DAA24388
AlphaFold O57683 Q99NB9 O75533 P59708 Q9Y3B4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks