BMRB Entry 11356

Title:
Solution structure of SH3 domain in Rac/Cdc42 guanine nucleotide exchange factor(GEF) 6
Deposition date:
2010-09-07
Original release date:
2011-09-07
Authors:
He, F.; Muto, Y.; Uda, H.; Koshiba, S.; Shirouzu, M.; Terada, T.; Kigawa, T.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Hirota, H.; Yoshida, M.; Kobayashi, N.; Tanaka, A.; Osanai, T.; Matsuo, Y.; Ohara, O.; Nagase, T.; Kikuno, R.; Nagayama, M.; Yokoyama, S.
Citation:

Citation: He, F.; Muto, Y.; Uda, H.; Koshiba, S.; Shirouzu, M.; Terada, T.; Kigawa, T.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Hirota, H.; Yoshida, M.; Kobayashi, N.; Tanaka, A.; Osanai, T.; Matsuo, Y.; Ohara, O.; Nagase, T.; Kikuno, R.; Nagayama, M.; Yokoyama, S.. "Solution structure of SH3 domain in Rac/Cdc42 guanine nucleotide exchange factor(GEF) 6"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P021021-16

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts72
1H chemical shifts462

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 76 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERHISGLN
2   LEUILEVALLYSALAARGPHEASNPHELYS
3   GLNTHRASNGLUASPGLULEUSERVALCYS
4   LYSGLYASPILEILETYRVALTHRARGVAL
5   GLUGLUGLYGLYTRPTRPGLUGLYTHRLEU
6   ASNGLYARGTHRGLYTRPPHEPROSERASN
7   TYRVALARGGLUILELYSSERSERGLUARG
8   SERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 0.8 mM; phosphate buffer NA 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.811, Kobayashi N. - data analysis

CYANA v1.0.7, Guntert P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA04985 BAC30599 BAF84431 BAH11929 BAH13153
EMBL CAD97632
GB AAH39856 AAH43505 AAI56698 AAM94903 AAU43636
REF NP_001005565 NP_001179929 NP_001253502 NP_001293106 NP_004831
SP Q15052 Q5XXR3 Q8K4I3
TPG DAA13327
AlphaFold Q8K4I3 Q5XXR3 Q15052

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks