BMRB Entry 11204

Title:
Solution structure of the second PDZ domain of harmonin protein
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second PDZ domain of harmonin protein"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: p040510-04

Data sets:
Data typeCount
13C chemical shifts463
15N chemical shifts107
1H chemical shifts738

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain of harmonin protein1

Entities:

Entity 1, PDZ domain of harmonin protein 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERPROGLY
2   ASNARGGLUASNLYSGLULYSLYSVALPHE
3   ILESERLEUVALGLYSERARGGLYLEUGLY
4   CYSSERILESERSERGLYPROILEGLNLYS
5   PROGLYILEPHEILESERHISVALLYSPRO
6   GLYSERLEUSERALAGLUVALGLYLEUGLU
7   ILEGLYASPGLNILEVALGLUVALASNGLY
8   VALASPPHESERASNLEUASPHISLYSGLU
9   ALAVALASNVALLEULYSSERSERARGSER
10   LEUTHRILESERILEVALALAALAALAGLY
11   ARGGLULEUPHEMETTHRASPARGSERGLY
12   PROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks