BMRB Entry 11151

Title:
Solution structure of the first Phorbol esters/diacylglycerol binding domain of human Protein kinase C, delta
Deposition date:
2010-04-15
Original release date:
2011-05-05
Authors:
Abe, H.; Miyamoto, K.; Tochio, N.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the first Phorbol esters/diacylglycerol binding domain of human Protein kinase C, delta"  .

Assembly members:

Assembly members:
Phorbol esters/diacylglycerol binding domain (C1 domain), polymer, 83 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050302-69

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phorbol esters/diacylglycerol binding domain (C1 domain): GSSGSSGKQAKIHYIKNHEF IATFFGQPTFCSVCKDFVWG LNKQGYKCRQCNAAIHKKCI DKIIGRCTGTAANSRDTSGP SSG

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts68
1H chemical shifts483

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Phorbol esters/diacylglycerol binding domain (C1 domain)1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, Phorbol esters/diacylglycerol binding domain (C1 domain) 83 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSGLNALA
2   LYSILEHISTYRILELYSASNHISGLUPHE
3   ILEALATHRPHEPHEGLYGLNPROTHRPHE
4   CYSSERVALCYSLYSASPPHEVALTRPGLY
5   LEUASNLYSGLNGLYTYRLYSCYSARGGLN
6   CYSASNALAALAILEHISLYSLYSCYSILE
7   ASPLYSILEILEGLYARGCYSTHRGLYTHR
8   ALAALAASNSERARGASPTHRSERGLYPRO
9   SERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Phorbol esters/diacylglycerol binding domain (C1 domain), [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks