BMRB Entry 11148

Title:
Solution structure of the TIG domain from Human Nuclear factor of activated T-cells, cytoplasmic 4
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the TIG domain from Human Nuclear factor of activated T-cells, cytoplasmic 4"  .

Assembly members:

Assembly members:
TIG domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P061030-04

Data sets:
Data typeCount
13C chemical shifts509
15N chemical shifts119
1H chemical shifts795

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TIG domain1

Entities:

Entity 1, TIG domain 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEUPROGLN
2   VALGLUALATYRSERPROSERALACYSSER
3   VALARGGLYGLYGLUGLULEUVALLEUTHR
4   GLYSERASNPHELEUPROASPSERLYSVAL
5   VALPHEILEGLUARGGLYPROASPGLYLYS
6   LEUGLNTRPGLUGLUGLUALATHRVALASN
7   ARGLEUGLNSERASNGLUVALTHRLEUTHR
8   LEUTHRVALPROGLUTYRSERASNLYSARG
9   VALSERARGPROVALGLNVALTYRPHETYR
10   VALSERASNGLYARGARGLYSARGSERPRO
11   THRGLNSERPHEARGPHELEUPROVALILE
12   CYSLYSGLUGLU

Samples:

sample_1: TIG domain, [U-13C; U-15N], 0.94 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE II 900 MHz

Related Database Links:

PDB
DBJ BAG56726 BAG63617 BAI46039
GB AAA79175 AAH08857 AAH53855 ABZ92167 ACG55652
REF NP_001129494 NP_001185894 NP_001185895 NP_001185896 NP_001275731
SP Q14934
AlphaFold Q14934

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks