BMRB Entry 11046

Title:
hsRad51-bound ssDNA
Deposition date:
2008-05-15
Original release date:
2009-10-15
Authors:
Shibata, Takehiko; Ito, Yutaka
Citation:

Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems."  J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448

Assembly members:

Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
hsRad51, polymer, 339 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Data sets:
Data typeCount
1H chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*DTP*DAP*DCP*DG)-3') 11
2DNA (5'-D(*DTP*DAP*DCP*DG)-3') 21
3DNA (5'-D(*DTP*DAP*DCP*DG)-3') 31
4DNA (5'-D(*DTP*DAP*DCP*DG)-3') 41
5DNA (5'-D(*DTP*DAP*DCP*DG)-3') 51
6DNA (5'-D(*DTP*DAP*DCP*DG)-3') 61
7DNA (5'-D(*DTP*DAP*DCP*DG)-3') 71
8DNA (5'-D(*DTP*DAP*DCP*DG)-3') 81
9DNA (5'-D(*DTP*DAP*DCP*DG)-3') 91
10DNA (5'-D(*DTP*DAP*DCP*DG)-3') 101
11hsRad512

Entities:

Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.

1   DTDADCDG

Entity 2, hsRad51 339 residues - Formula weight is not available

1   METALAMETGLNMETGLNLEUGLUALAASN
2   ALAASPTHRSERVALGLUGLUGLUSERPHE
3   GLYPROGLNPROILESERARGLEUGLUGLN
4   CYSGLYILEASNALAASNASPVALLYSLYS
5   LEUGLUGLUALAGLYPHEHISTHRVALGLU
6   ALAVALALATYRALAPROLYSLYSGLULEU
7   ILEASNILELYSGLYILESERGLUALALYS
8   ALAASPLYSILELEUALAGLUALAALALYS
9   LEUVALPROMETGLYPHETHRTHRALATHR
10   GLUPHEHISGLNARGARGSERGLUILEILE
11   GLNILETHRTHRGLYSERLYSGLULEUASP
12   LYSLEULEUGLNGLYGLYILEGLUTHRGLY
13   SERILETHRGLUMETPHEGLYGLUPHEARG
14   THRGLYLYSTHRGLNILECYSHISTHRLEU
15   ALAVALTHRCYSGLNLEUPROILEASPARG
16   GLYGLYGLYGLUGLYLYSALAMETTYRILE
17   ASPTHRGLUGLYTHRPHEARGPROGLUARG
18   LEULEUALAVALALAGLUARGTYRGLYLEU
19   SERGLYSERASPVALLEUASPASNVALALA
20   TYRALAARGALAPHEASNTHRASPHISGLN
21   THRGLNLEULEUTYRGLNALASERALAMET
22   METVALGLUSERARGTYRALALEULEUILE
23   VALASPSERALATHRALALEUTYRARGTHR
24   ASPTYRSERGLYARGGLYGLULEUSERALA
25   ARGGLNMETHISLEUALAARGPHELEUARG
26   METLEULEUARGLEUALAASPGLUPHEGLY
27   VALALAVALVALILETHRASNGLNVALVAL
28   ALAGLNVALASPGLYALAALAMETPHEALA
29   ALAASPPROLYSLYSPROILEGLYGLYASN
30   ILEILEALAHISALASERTHRTHRARGLEU
31   TYRLEUARGLYSGLYARGGLYGLUTHRARG
32   ILECYSLYSILETYRASPSERPROCYSLEU
33   PROGLUALAGLUALAMETPHEALAILEASN
34   ALAASPGLYVALGLYASPALALYSASP

Samples:

sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 0.5 mM; hsRad51 0.05 mM; AMP-PNP 10 mM; MgCl2 5 mM; NaCl 100 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
transferred NOESYsample_1isotropicsample_conditions_1
DQF-COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1
ROESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAA02718 BAA02961 BAA02962 BAA03189 BAB27489
EMBL CAA69384 CAG38796
GB AAC28561 AAD49705 AAF69145 AAH27384 AAI05459
PRF 2102359A
REF NP_001003043 NP_001039644 NP_001075493 NP_001102674 NP_001116653
SP O77507 P70099 Q06609 Q08297 Q2KJ94
TPG DAA25435
AlphaFold Q06609 P70099 Q08297 Q2KJ94 O77507