BMRB Entry 10099

Title:
Solution Structure of the First Fibronectin Type III domain of human KIAA1568 Protein
Deposition date:
2007-02-13
Original release date:
2008-08-15
Authors:
Li, H.; Kigawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Li, H.; Kigawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the First Fibronectin Type III domain of human KIAA1568 Protein"  .

Assembly members:

Assembly members:
Fibronectin Type III domain, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P021007-51

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts120
1H chemical shifts775

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA1568 Protein1

Entities:

Entity 1, KIAA1568 Protein 117 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSASNTYR
2   ASPLEUSERASPLEUPROGLYPROPROSER
3   LYSPROGLNVALTHRASPVALTHRLYSASN
4   SERVALTHRLEUSERTRPGLNPROGLYTHR
5   PROGLYTHRLEUPROALASERALATYRILE
6   ILEGLUALAPHESERGLNSERVALSERASN
7   SERTRPGLNTHRVALALAASNHISVALLYS
8   THRTHRLEUTYRTHRVALARGGLYLEUARG
9   PROASNTHRILETYRLEUPHEMETVALARG
10   ALAILEASNPROGLNGLYLEUSERASPPRO
11   SERPROMETSERASPPROVALARGTHRGLN
12   ASPSERGLYPROSERSERGLY

Samples:

sample_1: fibronectin type III domain, [U-13C; U-15N], 1.1 mM; phosphate 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.811, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_002802664 XP_008574551 XP_009196316 XP_011746215 XP_011746218

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks