BMRB Entry 10093

Title:
Solution structure of the second CUT domain of human Homeobox protein Cux-2
Deposition date:
2007-01-24
Original release date:
2008-08-14
Authors:
Nameki, N.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Nameki, N.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second CUT domain of human Homeobox protein Cux-2"  .

Assembly members:

Assembly members:
CUT domain, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040114-61

Data sets:
Data typeCount
13C chemical shifts432
15N chemical shifts108
1H chemical shifts693

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Homeobox protein Cux-21

Entities:

Entity 1, Homeobox protein Cux-2 101 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNTYRGLU
2   LEUTYRMETTYRARGGLUVALASPTHRLEU
3   GLULEUTHRARGGLNVALLYSGLULYSLEU
4   ALALYSASNGLYILECYSGLNARGILEPHE
5   GLYGLULYSVALLEUGLYLEUSERGLNGLY
6   SERVALSERASPMETLEUSERARGPROLYS
7   PROTRPSERLYSLEUTHRGLNLYSGLYARG
8   GLUPROPHEILEARGMETGLNLEUTRPLEU
9   SERASPGLNLEUGLYGLNALAVALGLYGLN
10   GLNPROGLYALASERSERGLYPROSERSER
11   GLY

Samples:

sample_1: CUT domain, [U-13C; U-15N], 0.3 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated_NOESYnot availablenot availablenot available
3D 13C-separated_NOESYnot availablenot availablenot available

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - data analysis

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks