BMRB Entry 10008

Title:
Solution structure of C-terminal fibronectin type III domain of mouse 1700129L13Rik protein
Deposition date:
2005-10-25
Original release date:
2006-11-06
Authors:
Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of C-terminal fibronectin type III domain of mouse 1700129L13Rik protein"  not known ., .-..

Assembly members:

Assembly members:
FN3 domain, polymer, 123 residues, 13038.744 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P031015-19

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts106
1H chemical shifts737

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FN3 domain1

Entities:

Entity 1, FN3 domain 123 residues - 13038.744 Da.

1   GLYSERSERGLYSERSERGLYPROGLYALA
2   PROSERTHRVALARGILESERLYSASNVAL
3   ASPGLYILEHISLEUSERTRPGLUPROPRO
4   THRSERPROSERGLYASNILELEUGLUTYR
5   SERALATYRLEUALAILEARGTHRALAGLN
6   METGLNASPASNPROSERGLNLEUVALPHE
7   METARGILETYRCYSGLYLEULYSTHRSER
8   CYSTHRVALTHRALAGLYGLNLEUALAASN
9   ALAHISILEASPTYRTHRSERARGPROALA
10   ILEVALPHEARGILESERALALYSASNGLU
11   LYSGLYTYRGLYPROALATHRGLNILEARG
12   TRPLEUGLNGLYASNSERLYSSERGLYPRO
13   SERSERGLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 200 mM; d-DTT 2 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 0.22 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121 - processing

NMRView v5.0.4 - data analysis

Kujira v0.8994 - data analysis

CYANA v2.0.17 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB24953
REF XP_006514073 XP_011241845 XP_011241846 XP_011241847 XP_011241848
SP Q9D968
AlphaFold Q9D968

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks