BMRB Entry 7222

Title:
Amide chemical shifts of free and hyaluronan-bound Link_TSG6 at a range of pH values
Deposition date:
2006-07-12
Original release date:
2007-03-06
Authors:
Blundell, Charles; Day, Anthony
Citation:

Citation: Blundell, Charles; Mahoney, David; Cordell, Martin; Almond, Andrew; Kahmann, Jan; Perczel, Andras; Taylor, Jon; Campbell, Iain; Day, Anthony. "Determining the molecular basis for the pH-dependent interaction between the Link module of human TSG-6 and hyaluronan"  J. Biol. Chem. 282, 12976-12988 (2007).
PubMed: 17307731

Assembly members:

Assembly members:
Link_TSG6, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data typeCount
15N chemical shifts1080
1H chemical shifts1412

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Link_TSG61

Entities:

Entity 1, Link_TSG6 98 residues - Formula weight is not available

1   GLYVALTYRHISARGGLUALAARGSERGLY
2   LYSTYRLYSLEUTHRTYRALAGLUALALYS
3   ALAVALCYSGLUPHEGLUGLYGLYHISLEU
4   ALATHRTYRLYSGLNLEUGLUALAALAARG
5   LYSILEGLYPHEHISVALCYSALAALAGLY
6   TRPMETALALYSGLYARGVALGLYTYRPRO
7   ILEVALLYSPROGLYPROASNCYSGLYPHE
8   GLYLYSTHRGLYILEILEASPTYRGLYILE
9   ARGLEUASNARGSERGLUARGTRPASPALA
10   TYRCYSTYRASNPROHISALALYS

Samples:

sample_1: Link_TSG6, [U-15N], 1 ± 0.1 mM; H2O 90%; D2O 10%; sodium azide 0.02 %(w/v)

conditions_1: pH: 4.75; temperature: 298 K

conditions_2: pH: 5.0; temperature: 298 K

conditions_3: pH: 5.25; temperature: 298 K

conditions_4: pH: 5.5; temperature: 298 K

conditions_5: pH: 5.75; temperature: 298 K

conditions_6: pH: 6.0; temperature: 298 K

conditions_7: pH: 6.25; temperature: 298 K

conditions_8: pH: 6.5; temperature: 298 K

conditions_9: pH: 6.75; temperature: 298 K

conditions_10: pH: 7.0; temperature: 298 K

conditions_11: pH: 7.25; temperature: 298 K

conditions_12: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC_(example)sample_1not availablenot available

Software:

No software information available

NMR spectrometers:

  • GE Omega 500 MHz

Related Database Links:

BMRB 6392 6393 7221
PDB
DBJ BAE24204 BAJ20799
EMBL CAD12353 CAD13434 CAH74219
GB AAA03342 AAB00792 AAC53527 AAH21155 AAH30205
REF NP_001007814 NP_001075375 NP_001075780 NP_001252869 NP_009046
SP O08859 P98065 P98066
TPG DAA32671
AlphaFold P98066 O08859 P98065

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks