BMRB Entry 7139

Title:
1H,13C and 15N resonance assignments of barnase-barstar complex NMR deuterium methyl relaxation data for free barnase and barnase-barstar complex
Deposition date:
2006-05-22
Original release date:
2007-03-06
Authors:
Zhuravleva, Anastasia; Korzhnev, Dmitry; Nolde, Svetlana; Kay, Lewis; Arseniev, Alexander; Billiter, Martin; Orekhov, Vladislav
Citation:

Citation: Zhuravleva, Anastasia; Korzhnev, Dmitry; Nolde, Svetlana; Kay, Lewis; Arseniev, Alexander; Billiter, Martin; Orekhov, Vladislav. "Propagation of Dynamic Changes in Barnase Upon Binding of Barstar: An NMR and Computational Study"  J. Mol. Biol. 367, 1079-1092 (2007).
PubMed: 17306298

Assembly members:

Assembly members:
barnase, polymer, 110 residues, 12401.9 Da.

Natural source:

Natural source:   Common Name: Bacillus amyloliquefaciens   Taxonomy ID: 1390   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Bacillus amyloliquefaciens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
order parameters41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barnase1

Entities:

Entity 1, barnase 110 residues - 12401.9 Da.

1   ALAGLNVALILEASNTHRPHEASPGLYVAL
2   ALAASPTYRLEUGLNTHRTYRHISLYSLEU
3   PROASPASNTYRILETHRLYSSERGLUALA
4   GLNALALEUGLYTRPVALALASERLYSGLY
5   ASNLEUALAASPVALALAPROGLYLYSSER
6   ILEGLYGLYASPILEPHESERASNARGGLU
7   GLYLYSLEUPROGLYLYSSERGLYARGTHR
8   TRPARGGLUALAASPILEASNTYRTHRSER
9   GLYPHEARGASNSERASPARGILELEUTYR
10   SERSERASPTRPLEUILETYRLYSTHRTHR
11   ASPHISTYRGLNTHRPHETHRLYSILEARG

Samples:

sample_1: barnase, [U-13C; U-15N; U-40% 2H], 1.3 mM

conditions_1: ionic strength: 0.03 M; pH: 6.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
(H)CC(CO)TOCSYsample_1not availableconditions_1
H(CC)(CO)NH-TOCSYsample_1not availableconditions_1
13C HSQC-RQ(3Dz2-2)sample_1not availableconditions_1
13C HSQC-RQ(D+z+Dz+)sample_1not availableconditions_1
13C HSQC-RQ(Dz)sample_1not availableconditions_1
13C HSQC-RQ(D+)sample_1not availableconditions_1
15N HSQCsample_1not availableconditions_1

Software:

NMRPipe, Frank Delaglio -

CARA, . -

DASHA v4.1, Prof. A.S Arseniev -

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 16169 16170 16171 16172 2066 2067 2070 4964 975
PDB
EMBL CAA31365 CBI44445 CCF06756 CCG51396 CDG27389
GB AAA86441 AAB28825 AAB29635 AAC53661 AAP41137
PRF 1204204A 2004243A 721946A
REF WP_003151497 WP_007407419 WP_012118533 WP_013353722 WP_014472289
SP P00648 P35078
AlphaFold P35078 P00648