BMRB Entry 6789

Title:
Backbone 1H- and 15N-amide chemical shifts of apo-, (Ca2+)1- and (Ca2+)2-Eq04Q TR2C of bovine calmodulin, at 301K and pH 6.0.
Deposition date:
2005-08-24
Original release date:
2013-02-15
Authors:
Evenas, Johan; Malmendal, Anders; Thulin, Eva; Carlstrom, Goran; Forsen, Sture
Citation:

Citation: Evenas, Johan; Malmendal, Anders; Thulin, Eva; Carlstrom, Goran; Forsen, Sture. "Ca2+ binding and conformational changes in a calmodulin domain."  Biochemistry 37, 13744-13754 (1998).
PubMed: 9753463

Assembly members:

Assembly members:
TR2C, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts71
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E104Q TR2C1

Entities:

Entity 1, E104Q TR2C 73 residues - Formula weight is not available

1   METLYSASPTHRASPSERGLUGLUGLUILE
2   ARGGLUALAPHEARGVALPHEASPLYSASP
3   GLYASNGLYTYRILESERALAALAGLULEU
4   ARGHISVALMETTHRASNLEUGLYGLULYS
5   LEUTHRASPGLUGLUVALASPGLUMETILE
6   ARGGLUALAASPILEASPGLYASPGLYGLN
7   VALASNTYRGLUGLNPHEVALGLNMETMET
8   THRALALYS

Samples:

sample_1: TR2C, [U-15N], 1.16 mM; NaN3 0.1 mM; KOH 0.1 mM; HCl 0.1 mM

conditions_1: pH: 6.0; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
1H COSY DQsample_1not availableconditions_1
1H TOCSYsample_1not availableconditions_1
1H NOESYsample_1not availableconditions_1
2D 15N HSQCsample_1not availableconditions_1
1H-15N HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian UNITY PLUS 600 MHz

Related Database Links:

BMRB 11440 16994 17881 17981 17982 17983 18084 18323 19376 25344 7016 7017 7190
PDB
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566
GB AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961
REF XP_001687795 XP_001869425 XP_007442524 XP_010190430

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks