BMRB Entry 6784

Title:
KID domain
Deposition date:
2005-08-18
Original release date:
2005-10-19
Authors:
Radhakrishnan, Ishwar; Perez-Alvarado, Gabriela; Dyson, H.; Wright, Peter
Citation:

Citation: Radhakrishnan, Ishwar; Perez-Alvarado, Gabriela; Dyson, H.; Wright, Peter. "Conformational preferences in the Ser133-phosphorylated and non-phosphorylated forms of the kinase inducible transactivation domain of CREB"  FEBS Lett. 430, 317-322 (1998).
PubMed: 9688563

Assembly members:

Assembly members:
KID domain, polymer, 60 residues, 6822 Da.

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET-24a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts56
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KID1

Entities:

Entity 1, KID 60 residues - 6822 Da.

1   GLNILESERTHRILEALAGLUSERGLUASP
2   SERGLNGLUSERVALASPSERVALTHRASP
3   SERGLNLYSARGARGGLUILELEUSERARG
4   ARGPROSERTYRARGLYSILELEUASNASP
5   LEUSERSERASPALAPROGLYVALPROARG
6   ILEGLUGLUGLULYSSERGLUGLUGLUTHR

Samples:

sample_1: KID, [U-15N], 1.2 ± 0.1 mM; Tris-d11 20 mM; NaCL 50 mM; DTT 10 mM; NaN3 0.2%; H2O 90%; D2O 10%

sample_2: KID, [U-15N; U-13C], 1.2 ± 0.1 mM; Tris-d11 20 mM; NaCL 50 mM; DTT 10 mM; NaN3 0.2%; H2O 90%; D2O 10%

conditions_1: ionic strength: 50 mM; pH: 6.6; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availableconditions_1
HNCACBsample_2not availableconditions_1
CBCA(CO)HNsample_2not availableconditions_1
HNCOsample_2not availableconditions_1
HCACOsample_1not availableconditions_1
15N-TOCSY-HSQCsample_1not availableconditions_1
15N-NOESY-HSQCsample_1not availableconditions_1
13C-HSQCsample_2not availableconditions_1

Software:

Felix95 vFelix95, Molecular_Simulations_Inc.,_San_Diego -

Felix97 vFelix97, Molecular_Simulations_Inc.,_San_Diego -

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker AMX 500 MHz

Related Database Links:

BMRB 18695 6788
PDB
DBJ BAA36482 BAC32173 BAC32174 BAG11405 BAG35615
EMBL CAA32890 CAA39151 CAA40347 CAA42619 CAA42620
GB AAA35715 AAA35716 AAA35717 AAA37456 AAB20597
PIR A40120
PRF 1504306A
REF NP_001032815 NP_001041721 NP_001080072 NP_001093399 NP_001153276
SP P15337 P16220 P27925 Q01147
TPG DAA32469
AlphaFold P27925 P15337 P16220 Q01147

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks