BMRB Entry 6683

Title:
Backbone Resonance Assignments of Human Normal Adult Hemoglobin in the Deoxy Form
Deposition date:
2005-06-10
Original release date:
2007-06-05
Authors:
Sahu, Sarata; Simplaceanu, Virgil; Ho, Nancy; Giovannelli, Janel; Ho, Chien
Citation:

Citation: Sahu, Sarata; Simplaceanu, Virgil; Ho, Nancy; Giovannelli, Janel; Ho, Chien. "Backbone Resonance Assignment of Human Adult Hemoglobin in the Deoxy Form"  J. Biomol. NMR 36, 1-1 (2006).
PubMed: 16703421

Assembly members:

Assembly members:
deoxy-Hb A alpha, polymer, 141 residues, 64000 Da.
deoxy-Hb A beta, polymer, 146 residues, 64000 Da.
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts694
15N chemical shifts222
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1globin subunit alpha one1
2globin subunit alpha two1
3globin subunit beta one2
4globin subunit beta two2
5heme, 13
6heme, 23
7heme, 33
8heme, 43

Entities:

Entity 1, globin subunit alpha one 141 residues - 64000 Da.

1   VALLEUSERPROALAASPLYSTHRASNVAL
2   LYSALAALATRPGLYLYSVALGLYALAHIS
3   ALAGLYGLUTYRGLYALAGLUALALEUGLU
4   ARGMETPHELEUSERPHEPROTHRTHRLYS
5   THRTYRPHEPROHISPHEASPLEUSERHIS
6   GLYSERALAGLNVALLYSGLYHISGLYLYS
7   LYSVALALAASPALALEUTHRASNALAVAL
8   ALAHISVALASPASPMETPROASNALALEU
9   SERALALEUSERASPLEUHISALAHISLYS
10   LEUARGVALASPPROVALASNPHELYSLEU
11   LEUSERHISCYSLEULEUVALTHRLEUALA
12   ALAHISLEUPROALAGLUPHETHRPROALA
13   VALHISALASERLEUASPLYSPHELEUALA
14   SERVALSERTHRVALLEUTHRSERLYSTYR
15   ARG

Entity 2, globin subunit beta one 146 residues - 64000 Da.

1   VALHISLEUTHRPROGLUGLULYSSERALA
2   VALTHRALALEUTRPGLYLYSVALASNVAL
3   ASPGLUVALGLYGLYGLUALALEUGLYARG
4   LEULEUVALVALTYRPROTRPTHRGLNARG
5   PHEPHEGLUSERPHEGLYASPLEUSERTHR
6   PROASPALAVALMETGLYASNPROLYSVAL
7   LYSALAHISGLYLYSLYSVALLEUGLYALA
8   PHESERASPGLYLEUALAHISLEUASPASN
9   LEULYSGLYTHRPHEALATHRLEUSERGLU
10   LEUHISCYSASPLYSLEUHISVALASPPRO
11   GLUASNPHEARGLEULEUGLYASNVALLEU
12   VALCYSVALLEUALAHISHISPHEGLYLYS
13   GLUPHETHRPROPROVALGLNALAALATYR
14   GLNLYSVALVALALAGLYVALALAASNALA
15   LEUALAHISLYSTYRHIS

Entity 3, heme, 1 - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

deoxy-Hb_A_1: alpha_chain, [U-13C; U-15N; U-2H], 2 mM; beta_chain 2 mM; heme 4 mM

deoxy-Hb_A_2: alpha_chain 2 mM; beta_chain, [U-13C; U-15N; U-2H], 2 mM; heme 4 mM

deoxy-Hb_A_3: alpha_chain, [U-15N; U-2H], 2 mM; beta_chain 2 mM; heme 4 mM

deoxy-Hb_A_4: alpha_chain 2 mM; beta_chain, [U-15N; U-2H], 2 mM; heme 2 mM

conditions_1: ionic strength: 0.1 M; pH: 7; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
(1H_15N)-TROSY_HSQCnot availablenot availableconditions_1
TROSY-HNCAnot availablenot availableconditions_1
TROSY-HNCOCAnot availablenot availableconditions_1
TROSY-HNCACBnot availablenot availableconditions_1
TROSY-HN(CO)CACBnot availablenot availableconditions_1
TROSY-HNCOnot availablenot availableconditions_1
3D HSQC-NOESY-TROSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance_DRX 600 MHz

Related Database Links:

BMRB 1053 1102 2006 2708 2710 5856 6230 7125 908
PDB
DBJ BAG34767
EMBL CAA23756 CAA23758 CAA23759 CAA43421 CAG38767
GB AAA16334 AAA21100 AAA21101 AAA21102 AAA21103
PRF 0404170B 0907233B
REF NP_000509 XP_002822173 XP_003819077 XP_004050595 XP_004090697
SP P02024 P02025 P02032 P68871 P68872
AlphaFold P02024 P02025 P02032 P68871 P68872

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks