BMRB Entry 5666

Title:
1H, 15N and 13C backbone assignment of the Green Fluorescent Protein (GFP)
Deposition date:
2003-01-22
Original release date:
2003-06-10
Authors:
Khan, Farid; Stott, Katherine; Jackson, Sophie
Citation:

Citation: Khan, Farid; Stott, Katherine; Jackson, Sophie. "Letter to the Editor: 1H, 15N and 13C backbone assignment of the Green Fluorescent Protein (GFP)"  J. Biomol. NMR 26, 281-282 (2003).

Assembly members:

Assembly members:
Green Fluorescent Protein, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: jellyfish   Taxonomy ID: 6100   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aequorea victoria

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts588
15N chemical shifts199
1H chemical shifts199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GFP monomer1

Entities:

Entity 1, GFP monomer 229 residues - Formula weight is not available

1   GLYSERLYSGLYGLUGLULEUPHETHRGLY
2   VALVALPROILELEUVALGLULEUASPGLY
3   ASPVALASNGLYHISLYSPHESERVALSER
4   GLYGLUGLYGLUGLYASPALATHRTYRGLY
5   LYSLEUTHRLEULYSPHEILECYSTHRTHR
6   GLYLYSLEUPROVALPROTRPPROTHRLEU
7   VALTHRTHRPHESERTYRGLYVALGLNCYS
8   PHESERARGTYRPROASPHISMETLYSARG
9   HISASPPHEPHELYSSERALAMETPROGLU
10   GLYTYRVALGLNGLUARGTHRILESERPHE
11   LYSASPASPGLYASNTYRLYSTHRARGALA
12   GLUVALLYSPHEGLUGLYASPTHRLEUVAL
13   ASNARGILEGLULEULYSGLYILEASPPHE
14   LYSGLUASPGLYASNILELEUGLYHISLYS
15   LEUGLUTYRASNTYRASNSERHISASNVAL
16   TYRILETHRALAASPLYSGLNLYSASNGLY
17   ILELYSALAASNPHELYSILEARGHISASN
18   ILEGLUASPGLYSERVALGLNLEUALAASP
19   HISTYRGLNGLNASNTHRPROILEGLYASP
20   GLYPROVALLEULEUPROASPASNHISTYR
21   LEUSERTHRGLNSERALALEUSERLYSASP
22   PROASNGLULYSARGASPHISMETVALLEU
23   LEUGLUPHEVALTHRALAALAGLYILE

Samples:

sample_1: Green Fluorescent Protein, [U-95% 13C; U-95% 15N], 1.0 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 6.0 mM

sample_2: Green Fluorescent Protein, [U-95% 13C; U-95% 15N; U-75% 2H], 1.0 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 6.0 mM

Experimental_conditions: pH: 7.2 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 5514
PDB
DBJ BAA93576 BAE45350 BAG30881
EMBL CAA10279 CAA65278 CAB61435 CAB70975 CAC27829
GenBank AAA27721 AAA69542 AAA69544 AAA69546 AAA69548
SWISS-PROT P42212

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks