BMRB Entry 4338

Title:
Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex.
Deposition date:
1999-04-23
Original release date:
1999-05-10
Authors:
Guilhaudis, Laure; Simorre, Jean-Pierre; Bouchayer, Eftychia; Neuburger, Michel; Bourguignon, Jacques; Douce, Roland; Marion, Dominique; Gans, Pierre
Citation:

Citation: Guilhaudis, Laure; Simorre, Jean-Pierre; Bouchayer, Eftychia; Neuburger, Michel; Bourguignon, Jacques; Douce, Roland; Marion, Dominique; Gans, Pierre. "Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex."  J. Biomol. NMR 15, 185-186 (1999).
PubMed: 10605092

Assembly members:

Assembly members:
Glycine Cleavage System apoprotein H, polymer, 131 residues, 13948 Da.

Natural source:

Natural source:   Common Name: Garden Pea   Taxonomy ID: 3888   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pisum sativum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia Coli   Vector: pET-H

Entity Sequences (FASTA):

Data typeCount
15N chemical shifts119
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hapo subunit 11

Entities:

Entity 1, Hapo subunit 1 131 residues - 13948 Da.

1   SERASNVALLEUASPGLYLEULYSTYRALA
2   PROSERHISGLUTRPVALLYSHISGLUGLY
3   SERVALALATHRILEGLYILETHRASPHIS
4   ALAGLNASPHISLEUGLYGLUVALVALPHE
5   VALGLULEUPROGLUPROGLYVALSERVAL
6   THRLYSGLYLYSGLYPHEGLYALAVALGLU
7   SERVALLYSALATHRSERASPVALASNSER
8   PROILESERGLYGLUVALILEGLUVALASN
9   THRGLYLEUTHRGLYLYSPROGLYLEUILE
10   ASNSERSERPROTYRGLUASPGLYTRPMET
11   ILELYSILELYSPROTHRSERPROASPGLU
12   LEUGLUSERLEULEUGLYALALYSGLUTYR
13   THRLYSPHECYSGLUGLUGLUASPALAALA
14   HIS

Samples:

sample_one: Glycine Cleavage System apoprotein H, [U-99% 15N], 1.0 mM; potassium phosphate 50 mM; EDTA 0.1 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_conditions_one: pH: 5.5 na; temperature: 308 K

sample_conditions_two: pH: 5.5 na; temperature: 303 K

sample_conditions_three: pH: 5.5 na; temperature: 297 K

sample_conditions_four: pH: 5.5 na; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY-HSQCsample_onenot availablenot available
3D 1H-15N TOCSY-HSQCsample_onenot availablenot available
2D 1H-15N HSQCsample_onenot availablenot available

Software:

FELIX v95, 97 -

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 4336 4337
PDB
EMBL CAA37704 CAA45978 CAI79404 CAJ13415 CAJ13721
GB AAA33668
PRF 1923203A
SP P16048
AlphaFold P16048

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks