BMRB Entry 26636

Title:
1H and 15N Chemical Shifts of Intrinsically Disordered Dehydrin ERD14 at Various Temperature and pH Values
Deposition date:
2015-08-15
Original release date:
2015-09-15
Authors:
Szalaine Agoston, Bianka
Citation:

Citation: Murvai, Nikoletta; Kalmar, Lajos; Szalaine Agoston, Bianka; Szabo, Beata; Tantos, Agnes; Csikos, Gyorgy; Micsonai, Andras; Kardos, Jozsef; Vertommen, Didier; Nguyen, Phuong; Hristozova, Nevena; Lang, Andras; Kovacs, Denes; Buday, Laszlo; Han, Kyou-Hoon; Perczel, Andras; Tompa, Peter. "Interplay of Structural Disorder and Short Binding Elements in the Cellular Chaperone Function of Plant Dehydrin ERD14"  Cells 9, 1856-1856 (2020).
PubMed: 32784707

Assembly members:

Assembly members:
ERD14, polymer, 185 residues, 20786.3 Da.

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Data typeCount
15N chemical shifts916
1H chemical shifts916

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ERD141

Entities:

Entity 1, ERD14 185 residues - 20786.3 Da.

1   METALAGLUGLUILELYSASNVALPROGLU
2   GLNGLUVALPROLYSVALALATHRGLUGLU
3   SERSERALAGLUVALTHRASPARGGLYLEU
4   PHEASPPHELEUGLYLYSLYSLYSASPGLU
5   THRLYSPROGLUGLUTHRPROILEALASER
6   GLUPHEGLUGLNLYSVALHISILESERGLU
7   PROGLUPROGLUVALLYSHISGLUSERLEU
8   LEUGLULYSLEUHISARGSERASPSERSER
9   SERSERSERSERSERGLUGLUGLUGLYSER
10   ASPGLYGLULYSARGLYSLYSLYSLYSGLU
11   LYSLYSLYSPROTHRTHRGLUVALGLUVAL
12   LYSGLUGLUGLULYSLYSGLYPHEMETGLU
13   LYSLEULYSGLULYSLEUPROGLYHISLYS
14   LYSPROGLUASPGLYSERALAVALALAALA
15   ALAPROVALVALVALPROPROPROVALGLU
16   GLUALAHISPROVALGLULYSLYSGLYILE
17   LEUGLULYSILELYSGLULYSLEUPROGLY
18   TYRHISPROLYSTHRTHRVALGLUGLUGLU
19   LYSLYSASPLYSGLU

Samples:

sample_1: ERD14, [U-15N], 1 mM; DSS 1 mM; sodium phosphate 32 mM; potassium phosphate 22 mM; sodium chloride 8.6 mM; calcium chloride 0.1 mM; magnesium sulfate 1 mM; carbenicillin 50 mg/L; glucose.H2O 4 g/L; H2O 90%; D2O 10%

288K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 288 K

282.5K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 282.5 K

277K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 277 K

277K-6.94pH: pH: 6.94; pressure: 1 atm; temperature: 277 K

277K-7.29pH: pH: 7.29; pressure: 1 atm; temperature: 277 K

277K-7.42pH: pH: 7.42; pressure: 1 atm; temperature: 277 K

277K-7.57pH: pH: 7.57; pressure: 1 atm; temperature: 277 K

277K-7.70pH: pH: 7.70; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropic288K-6.58pH
2D 1H-15N HSQCsample_1isotropic282.5K-6.58pH
2D 1H-15N HSQCsample_1isotropic277K-6.58pH
2D 1H-15N HSQCsample_1isotropic277K-6.94pH
2D 1H-15N HSQCsample_1isotropic277K-7.29pH
2D 1H-15N HSQCsample_1isotropic277K-7.42pH
2D 1H-15N HSQCsample_1isotropic277K-7.57pH
2D 1H-15N HSQCsample_1isotropic277K-7.70pH

Software:

CARA v1.8.4.2, Rochus Keller - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

UNP P42763
AlphaFold Q9C5R5 P42763

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks