BMRB Entry 26630

Title:
Protein G Domain Beta-1 Wild Type
Deposition date:
2015-08-10
Original release date:
2015-09-29
Authors:
Davey, James; Damry, Adam; Euler, Christian; Goto, Natalie; Chica, Roberto
Citation:

Citation: Davey, James; Damry, Adam; Euler, Christian; Goto, Natalie; Chica, Roberto. "Prediction of Stable Globular Proteins Using Negative Design with Non-native Backbone Ensembles"  Structure 23, 2011-2021 (2015).
PubMed: 26412333

Assembly members:

Assembly members:
Protein_G_Domain_Beta-1_Wild_Type, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1325   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein_G_Domain_Beta-1_Wild_Type: MHHHHHHGMTYKLILNGKTL KGETTTEAVDAATAEKVFKQ YANDNGVDGEWTYDDATKTF TVTE

Data sets:
Data typeCount
13C chemical shifts165
15N chemical shifts56
1H chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein G Domain Beta-1 Wild-Type1

Entities:

Entity 1, Protein G Domain Beta-1 Wild-Type 64 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYMETTHR
2   TYRLYSLEUILELEUASNGLYLYSTHRLEU
3   LYSGLYGLUTHRTHRTHRGLUALAVALASP
4   ALAALATHRALAGLULYSVALPHELYSGLN
5   TYRALAASNASPASNGLYVALASPGLYGLU
6   TRPTHRTYRASPASPALATHRLYSTHRPHE
7   THRVALTHRGLU

Samples:

sample_1: Protein G Domain Beta-1 Wild Type, [U-99% 13C; U-98% 15N], 0.5 – 2 mM; D2O, [U-99.9% 2H], 10%; H2O 90%; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks